Document Detail

Nanoproteomics Enabling Personalized Nanomedicine.
MedLine Citation:
PMID:  22820526     Owner:  NLM     Status:  Publisher    
Nucleic Acid Programmable Protein Arrays utilize a complex mammalian cell free expression system to produce proteins in situ. In alternative to fluorescent-labelled approaches a new label free method, emerging from the combined utilization of three independent and complementary nanotechnological approaches, appears capable to analyze protein function and protein-protein interaction in studies promising for Personalized Medicine. Quartz Micro Circuit nanogravimetry, based on frequency and dissipation factor, mass spectrometry and anodic porous alumina overcome indeed the limits of correlated fluorescence detection plagued by the background still present after extensive washes. This could be further optimized by an homogeneous and well defined bacterial cell free expression system capable to realize the ambitious objective to quantify the regulatory protein networks in humans. Implications for Personalized Medicine of the above Label Free protein array using different test genes proteins are reported.
Claudio Nicolini; Nicola Bragazzi; Eugenia Pechkova
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-19
Journal Detail:
Title:  Advanced drug delivery reviews     Volume:  -     ISSN:  1872-8294     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8710523     Medline TA:  Adv Drug Deliv Rev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Nanobiotechnology and Biophysics Laboratories, Department Experimental Medicine, University of Genova, Italy; Nanoworld Institute Fondazione EL.B.A Nicolini Bergamo, Italy; Biodesign Institute, Arizona State University, Tempe, USA.
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