Document Detail


Nanoporous micro-element arrays for particle interception in microfluidic cell separation.
MedLine Citation:
PMID:  22763858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability to control cell-surface interactions in order to achieve binding of specific cell types is a major challenge for microfluidic immunoaffinity cell capture systems. In the majority of existing systems, the functionalized capture surface is constructed of solid materials, where flow stagnation at the solid-liquid interface is detrimental to the convection of cells to the surface. We study the use of ultra-high porosity (99%) nanoporous micro-posts in microfluidic channels for enhancing interception efficiency of particles in flow. We show using both modelling and experiment that nanoporous posts improve particle interception compared to solid posts through two distinct mechanisms: the increase of direct interception, and the reduction of near-surface hydrodynamic resistance. We provide initial validation that the improvement of interception efficiency also results in an increase in capture efficiency when comparing nanoporous vertically aligned carbon nanotube (VACNT) post arrays with solid PDMS post arrays of the same geometry. Using both bacteria (∼1 μm) and cancer cell lines (∼15 μm) as model systems, we found capture efficiency increases by 6-fold and 4-fold respectively. The combined model and experimental platform presents a new generation of nanoporous microfluidic devices for cell isolation.
Authors:
Grace D Chen; Fabio Fachin; Elena Colombini; Brian L Wardle; Mehmet Toner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-05
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-03     Completed Date:  2012-12-03     Revised Date:  2013-07-25    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  3159-67     Citation Subset:  IM    
Affiliation:
BioMEMS Resource Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Separation / instrumentation,  methods*
Dimethylpolysiloxanes / chemistry
Escherichia coli / isolation & purification
Humans
Microfluidic Analytical Techniques / instrumentation*,  methods
Nanotechnology / instrumentation*
Nanotubes, Carbon / chemistry
Particle Size
Porosity
Grant Support
ID/Acronym/Agency:
P41 EB002503/EB/NIBIB NIH HHS; P41 EB002503/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Nanotubes, Carbon; 63148-62-9/baysilon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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