Document Detail


Nanoparticles functionalized with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells.
MedLine Citation:
PMID:  19889453     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Superparamagnetic iron oxide nanoparticles (SPIONs) can substantially improve the sensitivity of magnetic resonance imaging (MRI). We propose that SPIONs could be used to target and image cancer cells if functionalized with recombinant single chain Fv antibody fragments (scFv). We tested our hypothesis by generating antibody-functionalized (abf) SPIONs using a scFv specific for carcinoembryonic antigen (CEA), an oncofoetal cell surface protein. SPIONs of different hydrodynamic diameter and surface chemistry were investigated and targeting was confirmed by ELISA, cellular iron uptake, confocal laser scanning microscopy (CLSM) and MRI. Results demonstrated that abf-SPIONs bound specifically to CEA-expressing human tumour cells, generating selective image contrast on MRI. In addition, we observed that the cellular interaction of the abf-SPIONs was influenced by hydrodynamic size and surface coating. The results indicate that abf-SPIONs have potential for cancer-specific MRI.
Authors:
Kim L Vigor; Panagiotis G Kyrtatos; Shane Minogue; Khuloud T Al-Jamal; Heide Kogelberg; Berend Tolner; Kostas Kostarelos; Richard H Begent; Quentin A Pankhurst; Mark F Lythgoe; Kerry A Chester
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-04
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1307-15     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier Ltd. All rights reserved.
Affiliation:
UCL Cancer Institute, Paul O'Gorman Building, 72 Huntley Street London WC1E 6DD, UK.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Colorectal Neoplasms / immunology*,  pathology*
Contrast Media / chemical synthesis*
Drug Delivery Systems / methods
Ferric Compounds / chemistry,  diagnostic use*
Humans
Image Enhancement / methods
Immunomagnetic Separation / methods*
Nanoparticles / chemistry*,  diagnostic use*,  ultrastructure
Recombinant Proteins / diagnostic use
Single-Chain Antibodies / chemistry,  diagnostic use*
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Ferric Compounds; 0/Recombinant Proteins; 0/Single-Chain Antibodies; 1309-37-1/ferric oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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