Document Detail


Nanoparticles for cell labeling.
MedLine Citation:
PMID:  20938522     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell based therapeutics are emerging as powerful regimens. To better understand the migration and proliferation mechanisms of implanted cells, a means to track cells in living subjects is essential, and to achieve that, a number of cell labeling techniques have been developed. Nanoparticles, with their superior physical properties, have become the materials of choice in many investigations along this line. Owing to inherent magnetic, optical or acoustic attributes, these nanoparticles can be detected by corresponding imaging modalities in living subjects at a high spatial and temporal resolution. These features allow implanted cells to be separated from host cells; and have advantages over traditional histological methods, as they permit non-invasive, real-time tracking in vivo. This review attempts to give a summary of progress in using nanotechnology to monitor cell trafficking. We will focus on direct cell labeling techniques, in which cells ingest nanoparticles that bear traceable signals, such as iron oxide or quantum dots. Ferritin and MagA reporter genes that can package endogenous iron or iron supplement into iron oxide nanoparticles will also be discussed.
Authors:
Ashwinkumar Bhirde; Jin Xie; Maggie Swierczewska; Xiaoyuan Chen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Review     Date:  2010-10-11
Journal Detail:
Title:  Nanoscale     Volume:  3     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-13     Completed Date:  2011-05-03     Revised Date:  2011-06-22    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  England    
Other Details:
Languages:  eng     Pagination:  142-53     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institute of Health (NIH), Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / chemistry
Dendritic Cells / immunology,  metabolism
Embryonic Stem Cells / chemistry
Fluorescein-5-isothiocyanate / chemistry
Genes, Reporter
Humans
Magnetic Resonance Imaging
Mice
Microscopy, Fluorescence
Nanoparticles / chemistry*,  diagnostic use,  toxicity
Chemical
Reg. No./Substance:
0/Biological Markers; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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