| Nanoparticle realgar powders induce apoptosis in U937 cells through caspase MAPK and mitochondrial pathways. | |
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MedLine Citation:
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PMID: 17615687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nanoparticle realgar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibitor (z-DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3, poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In addition, NRP increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor (SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis. |
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Authors:
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Xiao-Bo Wang; Hui-Yuan Gao; Bai-Ling Hou; Jian Huang; Rong-Gang Xi; Li-Jun Wu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of pharmacal research Volume: 30 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-07-09 Completed Date: 2007-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 653-8 Citation Subset: IM |
Affiliation:
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School of Traditional Chinese Medicines, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Arsenicals / pharmacology* Caspases / physiology* DNA Fragmentation / drug effects Dose-Response Relationship, Drug Humans MAP Kinase Signaling System / physiology* Mitochondria / physiology* Nanoparticles* Powders Proto-Oncogene Proteins c-bcl-2 / physiology Signal Transduction / physiology* Sulfides / pharmacology* U937 Cells bcl-2-Associated X Protein / physiology |
| Chemical | |
Reg. No./Substance:
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0/Arsenicals; 0/Powders; 0/Proto-Oncogene Proteins c-bcl-2; 0/Sulfides; 0/bcl-2-Associated X Protein; 56320-22-0/arsenic disulfide; EC 3.4.22.-/Caspases |
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