Document Detail

Nanoparticle immunoagglutination Rayleigh scatter assay to complement microparticle immunoagglutination Mie scatter assay in a microfluidic device.
MedLine Citation:
PMID:  21411297     Owner:  NLM     Status:  Publisher    
In this work, particle immunoagglutination assays for pathogen detection, utilizing light scattering measurements at a fixed angle from incident light delivery, are explored in both Rayleigh and Mie scatter regimes through scatter intensity simulations and compared to experimental results. The average size of immunoagglutinated particles obtained from microscope images correspond to the particle size parameter from simulations. Mie scatter measurements yield a greater signal increase with increasing pathogen concentration than Rayleigh scatter measurements, but with a non-monotonic relationship that is not observed in the Rayleigh scatter regime. These two similar yet distinctly different sources of information could easily be integrated into a single device through fabrication of a simple microfluidic device containing two y-channels, each for performing the respective light scattering measurement. Escherichia coli was used as a representative target, and detected in a microfluidic device down to a concentration of 1 colony forming units (CFU) per mL.
Brian C Heinze; Jeong-Yeol Yoon
Related Documents :
22329007 - The different designs of molecule logic gates.
22060517 - Strong collapse turbulence in a quintic nonlinear schrödinger equation.
22462957 - A cryogenic circulating advective multi-pass absorption cell.
21416477 - Influence of extremely low-frequency electric fields on the growth of vigna radiata see...
18794937 - Nonlinear interaction between two different photonic bandgaps of a hybrid photonic crys...
21934767 - Digital dual-rate burst-mode receiver for 10g and 1g coexistence in optical access netw...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-22
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  -     ISSN:  1873-4367     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-3-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Catanionic solid lipid nanoparticles carrying doxorubicin for inhibiting the growth of U87MG cells.
Next Document:  International adoption and child psychological vulnerability.