Document Detail

Nanomolar detection with high sensitivity microfluidic absorption cells manufactured in tinted PMMA for chemical analysis.
MedLine Citation:
PMID:  21315925     Owner:  NLM     Status:  In-Data-Review    
We describe a novel, cost effective and simple technique for the manufacture of high sensitivity absorption cells for microfluidic analytical systems. The cells are made from tinted polymethyl methacrylate (PMMA) in which microfluidic channels are fabricated. Two windows (typically 250μm thick, resulting in little optical power loss) are formed at either end of the channel through which light is coupled. Unwanted stray light from the emitter passes through a greater thickness of the tinted substrate (typically the length of the cell) and is preferentially absorbed. In effect, this creates a pin-hole configuration over the length of the absorption cell, providing improved performances (sensitivity, S/N ratios, baseline noise and limit of detection) when used as an absorption cell compared to clear substrates. The method is used to achieve a LOD of 20nM with a colourimetric iron assay and a LOD of 0.22milli-absorption units with a pH assay.
Cedric F A Floquet; Vincent J Sieben; Ambra Milani; Etienne P Joly; Iain R G Ogilvie; Hywel Morgan; Matthew C Mowlem
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Publication Detail:
Type:  Journal Article     Date:  2010-12-25
Journal Detail:
Title:  Talanta     Volume:  84     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  235-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
National Oceanography Centre, University of Southampton, Southampton SO14 3ZH, UK(1).
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