Document Detail


Nanometer-scale mapping and single-molecule detection with color-coded nanoparticle probes.
MedLine Citation:
PMID:  18305159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a method for single-molecule detection and biomolecular structural mapping based on dual-color imaging and automated colocalization of bioconjugated nanoparticle probes at nanometer precision. In comparison with organic dyes and fluorescent proteins, nanoparticle probes such as fluorescence energy-transfer nanobeads and quantum dots provide significant advantages in signal brightness, photostability, and multicolor-light emission. As a result, we have achieved routine two-color superresolution imaging and single-molecule detection with standard fluorescence microscopes and inexpensive digital color cameras. By using green and red nanoparticles to simultaneously recognize two binding sites on a single target, individual biomolecules such as nucleic acids are detected and identified without target amplification or probe/target separation. We also demonstrate that a powerful astrophysical method (originally developed to analyze crowded stellar fields) can be used for automated and rapid statistical analysis of nanoparticle colocalization signals. The ability to rapidly localize bright nanoparticle probes at nanometer precision has implications not only for ultrasensitive medical detection but also for structural mapping of molecular complexes in which individual components are tagged with color-coded nanoparticles.
Authors:
Amit Agrawal; Rajesh Deo; Geoffrey D Wang; May D Wang; Shuming Nie
Related Documents :
20601949 - A photoactivatable marker protein for pulse-chase imaging with superresolution.
12880349 - High-resolution multiphoton tomography of human skin with subcellular spatial resolutio...
7745599 - Three-dimensionally resolved nad(p)h cellular metabolic redox imaging of the in situ co...
19619479 - Spike inference from calcium imaging using sequential monte carlo methods.
10430549 - Measurement of drug distribution in vascular tissue using quantitative fluorescence mic...
10866979 - Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence mic...
22854449 - Spatial-carrier phase-shifting digital holography utilizing spatial frequency analysis ...
25006979 - Polar bears from space: assessing satellite imagery as a tool to track arctic wildlife.
8746529 - Magnetic resonance imaging of the normal mouse brain: comparison with histologic sections.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-27
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  105     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-05     Completed Date:  2008-04-18     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3298-303     Citation Subset:  IM    
Affiliation:
Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 101 Woodruff Circle, Suite 2001, Atlanta, GA 30322, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Algorithms
Color
Image Processing, Computer-Assisted
Molecular Probe Techniques*
Molecular Probes / chemistry*,  diagnostic use
Nanoparticles / chemistry*,  diagnostic use
Nanotechnology / methods
Grant Support
ID/Acronym/Agency:
P20 GM072069/GM/NIGMS NIH HHS; P20 GM072069/GM/NIGMS NIH HHS; R01 CA108468/CA/NCI NIH HHS; R01 CA108468/CA/NCI NIH HHS; R01 GM60562/GM/NIGMS NIH HHS; U54 CA119338/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Molecular Probes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Highly efficient and large-scale generation of functional dopamine neurons from human embryonic stem...
Next Document:  Centroid estimation in discrete high-dimensional spaces with applications in biology.