| Nanolitre droplet array for real time reverse transcription polymerase chain reaction. | |
| | |
MedLine Citation:
|
PMID: 21359328 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Recently, more and more effort has been put into the miniaturization of genetic tests such as quantitative PCR (qPCR), because it is no doubt a powerful tool for molecular diagnosis and quantitative biology. In this paper, we developed a low density nanolitre droplet array generated on a chemical modified silicon chip for gene quantification. Reliable and sensitive two step real time qRT-PCR assay for microRNA measurement was performed within 500 nL droplets. It has a dynamic range of six orders of magnitude, allowing for the quantification of microRNA input from 10(3) to 10(9) copies per reaction. We successfully applied the platform for quantitative measurement of mir-122 across five cultured cell lines. The minimum total RNA input was as low as 1 pg per assay, which showed great potential for gene quantification at single cell level. We envision the droplet based qPCR chip would be a universal and low-cost platform for gene quantification in ordinary biological laboratories. |
| | |
Authors:
|
Yunxia Zhang; Ying Zhu; Bo Yao; Qun Fang |
Related Documents
:
|
2568168 - Heterogeneous expression of erbb-2 messenger rna in human breast cancer. 8621248 - Infrequent alterations of the p15, p16, cdk4 and cyclin d1 genes in non-astrocytic huma... 16540738 - Epidermal growth factor receptor gene amplification and protein expression in glioblast... 21725018 - An abc-type cobalt transport system is essential for growth of sinorhizobium meliloti a... 1714738 - Preferential methylation of the ha-ras proto-oncogene by methylnitrosourea in rat mamma... 3019838 - The sequence and mom-transactivation function of the c gene of bacteriophage mu. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-2-28 |
Journal Detail:
|
Title: Lab on a chip Volume: - ISSN: 1473-0189 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-3-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China. yaobo08@zju.edu.cn fangqun@zju.edu.cn. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interaction of rac-[M(diimine)(3)](2+) (M = Co, Ni) complexes with CT DNA: role of 5,6-dmp ligand on...
Next Document: Wide-field single metal nanoparticle spectroscopy for high throughput localized surface plasmon reso...