| Nanoflow electrospinning serial femtosecond crystallography. | |
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MedLine Citation:
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PMID: 23090408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption. |
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Authors:
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Raymond G Sierra; Hartawan Laksmono; Jan Kern; Rosalie Tran; Johan Hattne; Roberto Alonso-Mori; Benedikt Lassalle-Kaiser; Carina Glöckner; Julia Hellmich; Donald W Schafer; Nathaniel Echols; Richard J Gildea; Ralf W Grosse-Kunstleve; Jonas Sellberg; Trevor A McQueen; Alan R Fry; Marc M Messerschmidt; Alan Miahnahri; M Marvin Seibert; Christina Y Hampton; Dmitri Starodub; N Duane Loh; Dimosthenis Sokaras; Tsu-Chien Weng; Petrus H Zwart; Pieter Glatzel; Despina Milathianaki; William E White; Paul D Adams; Garth J Williams; Sébastien Boutet; Athina Zouni; Johannes Messinger; Nicholas K Sauter; Uwe Bergmann; Junko Yano; Vittal K Yachandra; Michael J Bogan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-10-18 |
Journal Detail:
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Title: Acta crystallographica. Section D, Biological crystallography Volume: 68 ISSN: 1399-0047 ISO Abbreviation: Acta Crystallogr. D Biol. Crystallogr. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-23 Completed Date: 2013-03-01 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 9305878 Medline TA: Acta Crystallogr D Biol Crystallogr Country: United States |
Other Details:
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Languages: eng Pagination: 1584-7 Citation Subset: IM |
Affiliation:
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PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Crystallization Crystallography, X-Ray / economics, instrumentation* Electromagnetic Fields Equipment Design Kinetics Lasers Sample Size Thermolysin / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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GM 55302/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.4.24.27/Thermolysin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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