Document Detail


Nanoflow electrospinning serial femtosecond crystallography.
MedLine Citation:
PMID:  23090408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
Authors:
Raymond G Sierra; Hartawan Laksmono; Jan Kern; Rosalie Tran; Johan Hattne; Roberto Alonso-Mori; Benedikt Lassalle-Kaiser; Carina Glöckner; Julia Hellmich; Donald W Schafer; Nathaniel Echols; Richard J Gildea; Ralf W Grosse-Kunstleve; Jonas Sellberg; Trevor A McQueen; Alan R Fry; Marc M Messerschmidt; Alan Miahnahri; M Marvin Seibert; Christina Y Hampton; Dmitri Starodub; N Duane Loh; Dimosthenis Sokaras; Tsu-Chien Weng; Petrus H Zwart; Pieter Glatzel; Despina Milathianaki; William E White; Paul D Adams; Garth J Williams; Sébastien Boutet; Athina Zouni; Johannes Messinger; Nicholas K Sauter; Uwe Bergmann; Junko Yano; Vittal K Yachandra; Michael J Bogan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-10-18
Journal Detail:
Title:  Acta crystallographica. Section D, Biological crystallography     Volume:  68     ISSN:  1399-0047     ISO Abbreviation:  Acta Crystallogr. D Biol. Crystallogr.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-03-01     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  9305878     Medline TA:  Acta Crystallogr D Biol Crystallogr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1584-7     Citation Subset:  IM    
Affiliation:
PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
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MeSH Terms
Descriptor/Qualifier:
Crystallization
Crystallography, X-Ray / economics,  instrumentation*
Electromagnetic Fields
Equipment Design
Kinetics
Lasers
Sample Size
Thermolysin / chemistry
Grant Support
ID/Acronym/Agency:
GM 55302/GM/NIGMS NIH HHS; R01 GM055302/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.24.27/Thermolysin
Comments/Corrections

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