Document Detail


Nanoencapsulation method for high selectivity sensing of hydrogen peroxide inside live cells.
MedLine Citation:
PMID:  20163178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reactive oxygen species (ROS) are ubiquitous in life and death processes of cells (Finkel, T.; Holbrook, N. J. Nature 2000, 408 (6809), 239-247), with a major role played by the most stable ROS, hydrogen peroxide (H(2)O(2)). However, the study of H(2)O(2) in live cells has been hampered by the absence of selective probes. Described here is a novel nanoprobe ("nanoPEBBLE") with dramatically improved H(2)O(2) selectivity. The traditional molecular probe, 2',7'-dichlorofluorescin (DCFH), which is also sensitive to most other ROS, was empowered with high selectivity by a nanomatrix that blocks the interference from all other ROS (hydroxyl radical, superoxide, nitric oxide, peroxynitrite, hypochlorous acid, and alkylperoxyl radical), as well as from enzymes such as peroxidases. The blocking is based on the combination of multiple exclusion principles: time barrier, hydrophobic energy barrier, and size barrier. However, H(2)O(2) sensitivity is maintained down to low nanomolar concentrations. The surface of the nanoprobe was engineered to address biological applications, and the power of this new nanoPEBBLE is demonstrated by its use on RAW264.7 murine macrophages. These nanoprobes may provide a powerful chemical detection/imaging tool for investigating biological mechanisms related to H(2)O(2) or other species, with high spatial and temporal resolution.
Authors:
Gwangseong Kim; Yong-Eun Koo Lee; Hao Xu; Martin A Philbert; Raoul Kopelman
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Publication Detail:
Type:  Evaluation Studies; Letter; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  82     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-12     Completed Date:  2010-06-08     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2165-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / methods*
Cell Line
Fluoresceins / analysis
Hydrogen Peroxide / analysis*
Macrophages / cytology*
Nanotechnology / methods*
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
N0-1-CO-37123/CO/NCI NIH HHS; N01 CO037123/CO/NCI NIH HHS; R01 ES008846/ES/NIEHS NIH HHS; T32 ES007062/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Fluoresceins; 2044-85-1/diacetyldichlorofluorescein; BBX060AN9V/Hydrogen Peroxide
Comments/Corrections

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