| Nanodiamond and nanoplatinum liquid, DPV576, activates human monocyte-derived dendritic cells in vitro. | |
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MedLine Citation:
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PMID: 21036722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The influence of nanoparticles on the immune system is poorly understood. It was recently shown that exposure to a mixture of nanodiamond (ND)- and nanoplatinum (NP)-coated material (DPV576-C) activates murine T-cells. This study examined the role of a dispersed aqueous mixture of ND/NP (DPV576) in activating human dendritic cells (DCs) in vitro. MATERIALS AND METHODS: Human monocyte-derived DCs were treated with DPV576 at various concentrations (50, 100 and 200 μg/ml) for 24 hours in vitro. Activation of DCs was determined by assessing the expression of co-stimulatory and maturation markers (CD80, CD83, CD86, HLADR), production of cytokines, and induction of proliferation of naïve CD4 T-cells. Expression of co-stimulatory molecules and cell proliferation were analysed by flow cytometry and cytokine secretion by ELISA. RESULTS: DPV576 treatment of DCs resulted in: (i) increased CD83 and CD86 expression on DCs, (ii) up-regulation in the levels of DC-secreted cytokines IL-6, TNF and IL-10, and (iii) increased ability to induce proliferation in CD4(+) T-cells which is associated with increased expression of T-cell activation marker CD25. CONCLUSION: Solution containing ND/NP (DPV576) activated human DCs and DCs-driven CD4 naive T-cell proliferation in vitro, which may be useful in boosting immune responses in cancer treatment. |
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Authors:
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Mamdooh Ghoneum; Alia Ghoneum; James Gimzewski |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-11-01 Completed Date: 2010-12-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 4075-9 Citation Subset: IM |
Affiliation:
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Charles Drew University of Medicine and Science, Department of Otolaryngology, Los Angeles, California 90059, USA. mghoneum@ucla.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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CD4-Positive T-Lymphocytes
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drug effects,
immunology Cells, Cultured Coated Materials, Biocompatible / administration & dosage* Cytokines / biosynthesis, immunology Dendritic Cells / cytology, drug effects*, immunology Humans Interleukin-2 Receptor alpha Subunit / biosynthesis, immunology Lymphocyte Activation / drug effects Metal Nanoparticles / administration & dosage* Monocytes / cytology, drug effects, immunology Nanodiamonds / administration & dosage* Platinum / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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0/Coated Materials, Biocompatible; 0/Cytokines; 0/IL2RA protein, human; 0/Interleukin-2 Receptor alpha Subunit; 0/Nanodiamonds; 7440-06-4/Platinum |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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