Document Detail

Nano titanium dioxide induces the generation of ROS and potential damage in HaCaT cells under UVA irradiation.
MedLine Citation:
PMID:  21121359     Owner:  NLM     Status:  MEDLINE    
Nano titanium dioxide (nano-TiO2) is frequently used in cosmetics, especially in sunscreen. Nano-TiO2 has been reported to be an efficient photocatalyst, which is able to produce reactive oxygen species (ROS) under UVA irradiation. However, the effects and mechanisms of skin toxicity caused by nano-TiO2 remain unclear. In this study, we explored the cytotoxicity and oxidative stress induced by nano-TiO2 under UVA irradiation with different crystal forms (anatase, rutile and anatase/rutile) and sizes (4 nm, 10 nm, 21 nm, 25 nm, 60 nm) in human keratinocyte HaCaT cells. Intracellular distribution of nano-TiO2, cell viability, intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), super oxide dismutase (SOD) activity and Malondialdehyde (MDA) content were measured. The results showed that nano-TiO2 (10-200 microg/ml) significantly reduced cell viability in a dose-dependent manner in HaCaT cells. The cell viability was 76.9%, 60.2%, and 44.1% following nano-TiO2 (4 nm), nano-TiO2 (10 nm) and P25 treatment at the concentration of 200 microg/ml, respectively (P < 0.01). Nano-TiO2 induced ROS resulted in oxidative stress in these cells by reducing SOD and increasing MDA levels. The MMP of the cells was decreased significantly (P < 0.01) while the apoptosis rate was increased. Anatase and amorphous forms of nano-TiO2 showed higher cytotoxicity than the rutile form. The results indicated that nano-TiO2 could induce the generation of ROS and damage HaCaT cells under UVA irradiation.
Chengbin Xue; Jianhong Wu; Fengli Lan; Wei Liu; Xiangliang Yang; Fandian Zeng; Huibi Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nanoscience and nanotechnology     Volume:  10     ISSN:  1533-4880     ISO Abbreviation:  J Nanosci Nanotechnol     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-02     Completed Date:  2010-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088195     Medline TA:  J Nanosci Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8500-7     Citation Subset:  IM    
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
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MeSH Terms
Apoptosis / drug effects*
Cell Line
Cell Survival / drug effects
Flow Cytometry
Keratinocytes / drug effects*,  metabolism
Membrane Potential, Mitochondrial / drug effects
Metal Nanoparticles / chemistry,  toxicity*
Microscopy, Electron, Transmission
Oxidative Stress
Particle Size
Reactive Oxygen Species / metabolism*
Titanium / chemistry,  pharmacokinetics,  toxicity*
Ultraviolet Rays
Reg. No./Substance:
0/Reactive Oxygen Species; 13463-67-7/titanium dioxide; 7440-32-6/Titanium

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