| Nano titanium dioxide induces the generation of ROS and potential damage in HaCaT cells under UVA irradiation. | |
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MedLine Citation:
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PMID: 21121359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nano titanium dioxide (nano-TiO2) is frequently used in cosmetics, especially in sunscreen. Nano-TiO2 has been reported to be an efficient photocatalyst, which is able to produce reactive oxygen species (ROS) under UVA irradiation. However, the effects and mechanisms of skin toxicity caused by nano-TiO2 remain unclear. In this study, we explored the cytotoxicity and oxidative stress induced by nano-TiO2 under UVA irradiation with different crystal forms (anatase, rutile and anatase/rutile) and sizes (4 nm, 10 nm, 21 nm, 25 nm, 60 nm) in human keratinocyte HaCaT cells. Intracellular distribution of nano-TiO2, cell viability, intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), super oxide dismutase (SOD) activity and Malondialdehyde (MDA) content were measured. The results showed that nano-TiO2 (10-200 microg/ml) significantly reduced cell viability in a dose-dependent manner in HaCaT cells. The cell viability was 76.9%, 60.2%, and 44.1% following nano-TiO2 (4 nm), nano-TiO2 (10 nm) and P25 treatment at the concentration of 200 microg/ml, respectively (P < 0.01). Nano-TiO2 induced ROS resulted in oxidative stress in these cells by reducing SOD and increasing MDA levels. The MMP of the cells was decreased significantly (P < 0.01) while the apoptosis rate was increased. Anatase and amorphous forms of nano-TiO2 showed higher cytotoxicity than the rutile form. The results indicated that nano-TiO2 could induce the generation of ROS and damage HaCaT cells under UVA irradiation. |
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Authors:
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Chengbin Xue; Jianhong Wu; Fengli Lan; Wei Liu; Xiangliang Yang; Fandian Zeng; Huibi Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of nanoscience and nanotechnology Volume: 10 ISSN: 1533-4880 ISO Abbreviation: J Nanosci Nanotechnol Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-02 Completed Date: 2010-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088195 Medline TA: J Nanosci Nanotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 8500-7 Citation Subset: IM |
Affiliation:
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College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Cell Line Cell Survival / drug effects Flow Cytometry Histocytochemistry Humans Keratinocytes / drug effects*, metabolism Membrane Potential, Mitochondrial / drug effects Metal Nanoparticles / chemistry, toxicity* Microscopy, Electron, Transmission Oxidative Stress Particle Size Reactive Oxygen Species / metabolism* Titanium / chemistry, pharmacokinetics, toxicity* Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 13463-67-7/titanium dioxide; 7440-32-6/Titanium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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