| Nano-osteoimmunology as an important consideration in the design of future implants. | |
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MedLine Citation:
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PMID: 21530692 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The size of wear particles emanating from a prosthesis at interfaces is critical to the interfacial properties of the joint replacement and responses from the biological environment. Nanoscale particles in particular require investigation. This project aimed to evaluate the osteoimmunological response to nanoscale ultrahigh molecular weight polyethylene (UHMWPE) wear particles in vitro, including dendritic cells (DCs), macrophages, osteoclasts (OCs), cytokine secretion, and co-cultured OCs and osteoblasts (OBs). The wear particles generated from a constant-load knee prosthesis actuator were profiled using atomic force microscopy and fractionated into sizes of 0.05-0.2, 0.2-0.8, 0.8-1, 1-5 and 5-10 μm. The fractions were exposed to DCs isolated from mice spleen, human OCs, and co-cultured human OBs and OCs, and the effects of the particles on the cells were determined. Results revealed that exposure to nanoscale UHMWPE wear particles induced significant DC activation (p<0.05) and consequently increased cytokine interleukin (IL)-6 and IL-1β secretion (p<0.05). Exposure to nanoscale particles promoted OC maturation, resulting in the suppression of OB proliferation in OB and OC co-cultures. Therefore, the results of this study could contribute to a more mechanistic understanding of wear-debris-associated prosthesis failure. Furthermore, nanoscale UHMWPE wear particles should be considered as mediators of periprosthetic inflammation in the future development of biomaterials for joint replacement bearing surfaces. |
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Authors:
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Neha Pal; Be Quah; Paul N Smith; Laura L Gladkis; Heiko Timmers; Rachel W Li |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-20 |
Journal Detail:
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Title: Acta biomaterialia Volume: 7 ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-05-30 Completed Date: 2011-09-16 Revised Date: 2012-01-09 |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: England |
Other Details:
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Languages: eng Pagination: 2926-34 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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The Medical School, The Australian National University, Canberra, ACT 0200, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / metabolism Bone and Bones / immunology Cells, Cultured Coculture Techniques Dendritic Cells / cytology, immunology Humans Knee Prosthesis Macrophages / cytology, immunology Materials Testing Mice Mice, Inbred C57BL Nanoparticles / adverse effects Nanotechnology / methods* Particle Size Polyethylenes / metabolism Prosthesis Design* Prosthesis Failure* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Polyethylenes; 0/ultra-high molecular weight polyethylene |
| Comments/Corrections | |
Comment In:
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Nanomedicine (Lond). 2011 Dec;6(10):1665-7
[PMID:
22122580
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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