Document Detail


Nano-mechanical properties of living cells expressing constitutively active RhoA effectors.
MedLine Citation:
PMID:  21078298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Filamentous actin and myosin-II are major determinants of cell mechanics and are tightly regulated by a small guanosine triphosphatase, RhoA, and its downstream effectors. We examined the effects of constitutively active mutants of RhoA effectors, which have not been reported before, on cortical stiffness of living cells by using scanning probe microscopy, fluorescence microscopy, and truncated mutants of RhoA effectors labeled with a fluorescent protein. Our data indicated that expression of a constitutively active mutant of Dia1, a formin-family actin polymerizer, enhanced cortical stiffness and increased actin filament quantity in cells. Furthermore, expression of a constitutively active mutant of Rho-associated coiled-coil kinase, a myosin-II activator, softened the cell cortex but increased myosin-II activity. Our findings provide new insights into anomalous mechanics of cells, which is a topic of current interest in a variety of biological research fields.
Authors:
Kazushi Tamura; Takeomi Mizutani; Hisashi Haga; Kazushige Kawabata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-13
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  403     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-20     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  363-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan. tam@mail.sci.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Carrier Proteins / genetics
Mice
Microscopy, Fluorescence
Mutation
NIH 3T3 Cells
Nanostructures
Stress Fibers / physiology
Stress, Mechanical*
rho GTP-Binding Proteins / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Diap1 protein, mouse; EC 3.6.5.2/RhoA protein, mouse; EC 3.6.5.2/rho GTP-Binding Proteins

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