| Nano-hydroxyapatite particles induce apoptosis on MC3T3-E1 cells and tissue cells in SD rats. | |
| | |
MedLine Citation:
|
PMID: 22450902 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
While the advantages of nanomaterials are being increasingly recognized, their potential toxicity is drawing more and more attention and concern. In this study, we explore the toxicity mechanism of 20-30 nm rod-shaped hydroxyapatite (HA) nanoparticles in vitro and in vivo. The nanoparticles were prepared by precipitation and characterized by IR, XRD and TEM. Concentrations of 0 μg mL(-1), 10 μg mL(-1), 100 μg mL(-1), 1 mg mL(-1), and 10 mg mL(-1) were applied to the MC3T3-E1 cells for viability (MTT-test). Based on the characteristic differences of the two methods of cell death, the morphological features of the MC3T3-E1 cell line co-cultured with nano-hydroxyapatite (n-HA) (10 mg mL(-1)) for 24 h were also observed by TEM. Furthermore, important serum biochemical markers and histopathological examinations were used to evaluate the potential toxicological effect of n-HA on the major organs of SD rats injected intraperitoneally with n-HA (33.3 mg kg(-1) body weight). In the results, we found cell growth inhibition and apoptosis in MC3T3-E1 cells co-cultured with n-HA. Moreover, apoptosis but not necrosis was illustrated in liver and renal tissue by using histopathology slices and serum biochemical markers. It suggests that apoptosis may be the possible mechanism of n-HA toxicity and provides a better understanding of the biocompatibility of nanomaterials applied in human bone repair. |
| | |
Authors:
|
Liting Wang; Gang Zhou; Haifeng Liu; Xufeng Niu; Jingyun Han; Lisha Zheng; Yubo Fan |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-3-27 |
Journal Detail:
|
Title: Nanoscale Volume: - ISSN: 2040-3372 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-3-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101525249 Medline TA: Nanoscale Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, School of Biological Sciences and Medical Engineering, Beihang University, XueYuan Road No.37, Haidian District, Beijing, 100191, China. yubofan@buaa.edu.cn. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Impact of Centers for Medicare & Medicaid Services national coverage determination on erythropoiesis...
Next Document: Factors affecting the local control of stereotactic body radiotherapy for lung tumors including prim...