| Nano-glue: An Alternative Way to Display Cell-internalizing Peptide at the Spikes of Hepatitis B Virus Core Nanoparticles for Cell-targeting Delivery. | |
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MedLine Citation:
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PMID: 22775561 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cell-internalizing peptides (CIPs) can be used to mediate specific delivery of nanoparticles across cellular membrane. The objective of this study was to develop a display technique using hepatitis B virus (HBV) capsid-binding peptide as a 'nano-glue' to present CIPs on HBV nanoparticles for cell-targeting delivery. A CIP was selected from a phage display library and cross-linked specifically at the tips of the spikes of the HBV capsid nanoparticle via the 'nano-glue' by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). Fluorescent oligonucleotides packaged in the nanoparticles and the fluorescein molecules conjugated on the nanoparticles were delivered to cells by using this display technique. This study demonstrated a proof of principle for cell-targeting delivery via 'nano-glue' bioconjugation. |
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Authors:
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Khai Wooi Lee; Beng Ti Tey; Kok Lian Ho; Bimo A Tejo; Wen Siang Tan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-9 |
Journal Detail:
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Title: Molecular pharmaceutics Volume: - ISSN: 1543-8392 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101197791 Medline TA: Mol Pharm Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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