Document Detail

Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light.
MedLine Citation:
PMID:  17663520     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the apoptotic effect of photoexcited titanium dioxide (TiO(2)) nanoparticles in the presence of visible light on human hepatoma cell line (Bel 7402) and to study the underlying mechanism.
METHODS: Cerium-element-doped titanium dioxide nanoparticles were prepared by impregnation method. Bel 7402 human hepatoma cells were cultured in RPMI 1640 medium in a humidified incubator with 50 mL/L CO(2) at 37 centigrade. A 15 W fluorescent lamp with continuous wavelength light was used as light source in the photocatalytic test. Fluorescence morphology and agarose gel eletrophoresis pattern were performed to analyze apoptotic cells.
RESULTS: The Ce (IV)-doped TiO(2) nanoparticles displayed their superiority. The adsorption edge shifted to the 400-450 nm region. With visible light illuminated for 10 min, 10 microg/cm(3) Ce (IV)-doped TiO(2) induced micronuclei and significant apoptosis in 4 and 24 h, respectively. Hochest 33258 staining of the fixed cells revealed typical apoptotic structures (apoptotic bodies), agarose gel electrophoresis showed typical DNA ladder pattern in treated cells but not in untreated ones.
CONCLUSION: Ce (IV) doped TiO(2) nanoparticles can induce apoptosis of Bel 7402 human hepatoma cells in the presence of visible light.
Long Wang; Jian Mao; Gao-Hua Zhang; Ming-Jing Tu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  13     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-31     Completed Date:  2007-11-07     Revised Date:  2014-09-25    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4011-4     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Hepatocellular / metabolism,  pathology*
Cell Line, Tumor
Cerium / chemistry*
DNA Fragmentation
Electrophoresis, Agar Gel
Liver Neoplasms / metabolism,  pathology*
Metal Nanoparticles / chemistry*
Nanotechnology / methods*
Photochemistry / methods
Spectrometry, Fluorescence
Titanium / chemistry*
X-Ray Diffraction
Reg. No./Substance:
15FIX9V2JP/titanium dioxide; 30K4522N6T/Cerium; D1JT611TNE/Titanium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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