| Naked Eye Detection of Glucose in Urine Using Glucose Oxidase Immobilized Gold Nanoparticles. | |
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MedLine Citation:
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PMID: 21391552 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Colloidal gold is extensively used for molecular sensing because of the flexibilities it offers in terms of modification of the gold nanoparticle surface with a variety of functional groups using thiol chemistry. We describe a simple assay that allows the visual detection of glucose in aqueous samples and demonstrates its applicability by estimating glucose in urine. To enable the glucose detection, we functionalized the thiol capped gold nanoparticles with glucose oxidase, the enzyme specific to β-D glucose, using carbodiimide chemistry. The visible color change of the GOD-functionalized gold nanoparticles from red to blue on interaction with glucose is the principle applied here for the sensing of urine glucose level. The solution turns blue when the glucose concentration exceeds 100 μg/mL. The approach depicted here seems to be important, particularly in third world countries where high tech diagnostics aids are inaccessible to the bulk of the population. |
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Authors:
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Changerath Radhakumary; Kunnatheeri Sreenivasan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-10 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratory for Polymer Analysis, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology , Thiruvananthapuram 695012, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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