Document Detail


Na(+)-dependent transport of D-xylose by bovine intestinal brush border membrane vesicles (BBMV) is inhibited by various pentoses and hexoses.
MedLine Citation:
PMID:  11199210     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To detect whether pentoses and hexoses occurring in rumen bacteria or in hemicellulose ingested with feed and partly released in the small intestine have an affinity for the Na(+)-dependent glucose transporter of the bovine intestinal brush border membrane (BBM), we investigated whether these monosaccharides inhibit Na(+)-dependent transport of 14C-labelled D-xylose across the BBM using brush border membrane vesicles (BBMV) isolated from the mid-jejunum of cows. We used D-xylose as the transport substrate, because it has a low affinity for the Na(+)-dependent glucose transporter and thus its uptake into BBMV is more efficiently competitively inhibited by other sugars than that of D-glucose. D-Ribose, D-mannose and L-rhamnose occurring in rumen bacteria significantly inhibited Na(+)-dependent uptake of D-xylose into BBMV, but their inhibitory effect was less than that of D-glucose, D-xylose and phlorizin. This also applied to L-arabinose (and D-arabinose), which is, like D-xylose and D-galactose, a constituent of hemicellulose, and to 2-deoxy-D-glucose. Of all monosaccharides tested, only D-fructose did not affect Na(+)-dependent D-xylose transport. It is concluded that some pentoses and hexoses occurring in rumen bacteria (D-ribose, D-mannose and L-rhamnose) or hemicellulose (L-arabinose and D-xylose) have a low affinity for the Na(+)-dependent glucose transporter of the bovine BBM and may therefore be absorbed from the jejunum when released in the small intestine.
Authors:
E Scharrer; B Grenacher
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary medicine. A, Physiology, pathology, clinical medicine     Volume:  47     ISSN:  0931-184X     ISO Abbreviation:  J Vet Med A Physiol Pathol Clin Med     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-29     Completed Date:  2001-04-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  100955112     Medline TA:  J Vet Med A Physiol Pathol Clin Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  617-26     Citation Subset:  IM    
Affiliation:
Institute of Veterinary Physiology, University of Zurich, Winterthurerstr. 260, CH-8057, Zurich, Switzerland. scharrer@vetphys.unizh.ch
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive
Biological Transport, Active
Cattle / metabolism*
Hexoses / pharmacology*
Jejunum / metabolism*
Kinetics
Microvilli / metabolism
Monosaccharide Transport Proteins / drug effects*
Pentoses / pharmacology*
Xylose / antagonists & inhibitors,  metabolism*
Chemical
Reg. No./Substance:
0/Hexoses; 0/Monosaccharide Transport Proteins; 0/Pentoses; 0/Xylose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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