| Na(+) but not Cl(-) or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings. | |
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MedLine Citation:
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PMID: 19423186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutathione reductase (GR, EC 1,6.4.2) is an important reactive oxygen species-scavenging enzyme. The present study examined the relative importance of Na(+), Cl(-), and the osmotic component in NaCl-induced expression of Oryza sativa glutathione reductase (OsGR) genes in rice roots. Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome. The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl. Treatment with 150 mM NaCl and 150 mM NaNO(3) affected OsGR2 and OsGR3 induction similarly, which suggests that Na(+) but not Cl(-) is required for the NaCl-induced expression of OsGR2 and OsGR3. We also show that Na(+) but not Cl(-) is required for NaCl-enhanced GR activity and hydrogen peroxide (H(2)O(2)) production in rice roots. In addition to its component of ion toxicity, salt concentration in soil results in an osmotic effect. Here, we show that OsGR2 and OsGR3 expression, GR activity, and H(2)O(2) content were not affected at a concentration of mannitol iso-osmotic with 150 mM NaCl. NaCl-induced OsGR2 and OsGR3 in rice roots could be associated with Na(+) but not an osmotic component. |
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Authors:
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Chwan-Yang Hong; Yun-Yang Chao; Min-Yu Yang; Shih-Chueh Cho; Ching Huei Kao |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-07 |
Journal Detail:
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Title: Journal of plant physiology Volume: 166 ISSN: 1618-1328 ISO Abbreviation: J. Plant Physiol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-15 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882059 Medline TA: J Plant Physiol Country: Germany |
Other Details:
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Languages: eng Pagination: 1598-606 Citation Subset: IM |
Affiliation:
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Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chlorine
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pharmacology* Gene Expression / drug effects Genome, Plant Glutathione Reductase / genetics, metabolism* Hydrogen Peroxide / metabolism Oryza sativa / drug effects*, enzymology, metabolism Osmotic Pressure* Plant Proteins / genetics, metabolism* Plant Roots / drug effects, enzymology, metabolism RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Sodium / pharmacology* Sodium Chloride / pharmacology* Stress, Physiological / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Plant Proteins; 0/RNA, Messenger; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride; 7722-84-1/Hydrogen Peroxide; 7782-50-5/Chlorine; EC 1.8.1.7/Glutathione Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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