| NTP and PCr responses to hypoxia by hypothermic and normothermic respiring, superfused, neonatal rat cerebrocortical slices: an NMR spectroscopy study at 14.1 Tesla. | |
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MedLine Citation:
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PMID: 14753408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although mechanisms of hypothermic neuroprotection during oxygen deprivation have long been investigated, further characterizations of various molecular mechanisms are appropriate. Anticipating future studies of hypothermia and hypoxia/ischemia, we investigated the extent to which our ex vivo, NMR-based, superfused brain slice model might be helpful. (Slices are approximately 350 microm thick, with 18 slices per 8 mm NMR tube.) 31P NMR spectroscopic measurements were made of hypothermia-induced changes in high energy phosphates, while simultaneously monitoring and controlling tissue temperature, using 1H NMR, the high spectroscopic resolution available at 14.1 Tesla (600 MHz for protons), and a recently published protocol. NTP and PCr concentrations in healthy, well-oxygenated slices decreased to (55 +/- 15)% and (66 +/- 30)% of their respective values at 28.0 degrees C when warmed to 38.0 degrees C, in approximate agreement with earlier in vivo studies by others. During 30 min hypoxia NTP and PCr decreased to non-observable values, regardless of temperature. After reoxygenation, NTP and PCr recoveries as percentages of respective prehypoxia values were (63% +/- 16%; 70%) +/- 5%) for hypothermic slices (28.0 degrees C), and (46% +/- 13%; 41% +/- hypothermic neuroprotection during oxygen deprivation in this model, which appears suitable for use in further studies. |
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Authors:
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L Litt; K Hirai; V J Basus; T L James |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Acta neurochirurgica. Supplement Volume: 86 ISSN: 0065-1419 ISO Abbreviation: Acta Neurochir. Suppl. Publication Date: 2003 |
Date Detail:
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Created Date: 2004-02-02 Completed Date: 2004-03-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100962752 Medline TA: Acta Neurochir Suppl Country: Austria |
Other Details:
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Languages: eng Pagination: 71-4 Citation Subset: IM |
Affiliation:
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Department of Anesthesia, University of California, San Francisco, San Francisco, California 94143-0648, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Anoxia / metabolism* Cerebral Cortex / metabolism* Cold Temperature* Magnetic Resonance Spectroscopy Nucleotides / metabolism* Oxygen Consumption* Perfusion Phosphates / metabolism* Phosphocreatine / metabolism* Protons Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM34767/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nucleotides; 0/Phosphates; 0/Protons; 67-07-2/Phosphocreatine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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