Document Detail

Neuronal restrictive silencing factor silencing induces human amniotic fluid-derived stem cells differentiation into insulin-producing cells.
MedLine Citation:
PMID:  20942606     Owner:  NLM     Status:  In-Data-Review    
Islet cell replacement represents the most promising approach for the treatment of type I diabetes. However, it is limited by a shortage of pancreas donors. Here, we report that human amniotic fluid-derived stem cells (hAFSCs) can be induced to differentiate into functional insulin-producing cells by knocking down neuronal restrictive silencing factor (NRSF). In this study, lentiviral vectors were used to deliver small interference NRSF (siNRSF) RNA into hAFSCs. After infection with lentivirus containing siNRSF, hAFSCs were successfully induced to differentiate into insulin-producing cells. The differentiated siNRSF-hAFSCs expressed genes specific for islet cells, such as Pdx1, Hnf4α, Isl-1, Nkx6.1, Insulin, and Glut2. These cells also produced and released C-peptide in a glucose-responsive manner. These findings indicated that hAFSCs could be induced to differentiate into insulin-producing β-like cells by NRSF silencing.
Baowei Li; Sihan Wang; Hui Liu; Daqing Liu; Jing Zhang; Bowen Zhang; Hailei Yao; Yang Lv; Ruoyong Wang; Lin Chen; Wen Yue; Yanhua Li; Xuetao Pei
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Publication Detail:
Type:  Journal Article     Date:  2010-11-29
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1223-31     Citation Subset:  IM    
Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine , Beijing, China .
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