| NPC1L1 and SR-BI are involved in intestinal cholesterol absorption from small-size lipid donors. | |
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MedLine Citation:
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PMID: 18373109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the human intestinal content after a meal, cholesterol is dispersed in a complex mixture of emulsified droplets, vesicles, mixed micelles and precipitated material. The aim of this study was to determine the contribution of the main intestinal cholesterol transporters (NPC1L1, SR-BI) to the absorption processes, using different cholesterol-solubilizing donors. Cholesterol donors prepared with different taurocholate concentrations were added to an apical medium of differentiated TC7/Caco-2 cells. As the taurocholate concentrations increased, cholesterol donor size decreased (from 712 to 7 nm in diameter), which enhanced cholesterol absorption in a dose-dependent manner (38-fold). Two transport processes were observed: (1) absorption from large donors exhibited low-capacity transport with no noticeable transporter contribution; (2) efficient cholesterol absorption occurs from small lipid donors (<or=23 nm diameter), mainly due to NPC1L1 and SR-BI involvement. In addition, bile acids significantly increased mRNA and protein expression of NPC1L1, but not of SR-BI. In conclusion, bile acids present in the intestinal lumen and the micelles enhance intestinal cholesterol transport into the cell by two different regulatory processes: by reducing the lipid donor size, so that small-size mixed micelles can more easily access brush-border membrane transporters, and by increasing the expression level of the enterocyte NPC1L1. These mechanisms could account for the important inter-individual variations observed in cholesterol intestinal absorption. |
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Authors:
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Ziad Haikal; Barbara Play; Jean-François Landrier; Annie Giraud; Odette Ghiringhelli; Denis Lairon; Dominique Jourdheuil-Rahmani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-29 |
Journal Detail:
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Title: Lipids Volume: 43 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-22 Completed Date: 2008-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 401-8 Citation Subset: IM |
Affiliation:
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INRA, UMR1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, INSERM, U476, Univ Aix-Marseille 1, Univ Aix-Marseille 2, Faculté de Médecine, IPHM-IFR 125, 27 bld Jean Moulin, 13385 Marseille, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Bile Acids and Salts / physiology Cell Line Cholesterol, Dietary / metabolism* DNA Primers Gene Expression Regulation / physiology Humans Intestinal Absorption / physiology* Membrane Proteins / genetics, metabolism, physiology* Scavenger Receptors, Class B / genetics, metabolism, physiology* Solubility |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Cholesterol, Dietary; 0/DNA Primers; 0/Membrane Proteins; 0/NPC1L1 protein, human; 0/SCARB1 protein, human; 0/Scavenger Receptors, Class B |
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