| NPC-16, a novel naphthalimide-polyamine conjugate, induced apoptosis and autophagy in human hepatoma HepG2 cells and Bel-7402 cells. | |
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MedLine Citation:
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PMID: 20809291 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The antitumor effects and molecular mechanism of NPC-16, a novel naphthalimide-polyamine conjugate, were evaluated in HepG2 cells and Bel-7402 cells. Apoptosis and necrosis were evaluated by Annexin V-FITC detection kit, and autophagy by acridine orange and Lyso-Tracker Red staining. The change of mitochondrial transmembrane potential was measured using rhodamine 123 staining. The protein expression of Beclin 1, LC3 II and mTOR, p70S6 K, 14-3-3, caspase, and Bcl-2 family members was detected by immunofluorescence assays and Western Blot. Here, we elucidated the nature of cellular response of HepG2 cells and Bel-7402 cells to NPC-16 at IC(50). NPC-16 induced caspase-dependent apoptosis via the mitochondrial pathway and death receptor pathway in Bel-7402 cells. Differently, NPC-16 triggered HepG2 cells both apoptosis and autophagy, further autophagy facilitated cellular apoptosis. Furthermore, mTOR signal pathway was involved in NPC-16-mediated autophagy in HepG2 cells. Thus, NPC-16 may be useful as a potential template for investigation the molecular mechanism of naphthalimide-polyamine conjugate against hepatocellular carcinoma. |
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Authors:
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Song-Qiang Xie; Qian Li; Ya-Hong Zhang; Jian-Hong Wang; Zi-Hou Mei; Jin Zhao; Chao-Jie Wang |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Apoptosis : an international journal on programmed cell death Volume: 16 ISSN: 1573-675X ISO Abbreviation: Apoptosis Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
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Languages: eng Pagination: 27-34 Citation Subset: IM |
Affiliation:
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Institute of Chemical Biology, Henan University, Kaifeng, 475004, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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