Document Detail


NO3-/NO2- assimilation in halophilic archaea: physiological analysis, nasA and nasD expressions.
MedLine Citation:
PMID:  19593595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The haloarchaeon Haloferax mediterranei is able to assimilate nitrate or nitrite using the assimilatory nitrate pathway. An assimilatory nitrate reductase (Nas) and an assimilatory nitrite reductase (NiR) catalyze the first and second reactions, respectively. The genes involved in this process are transcribed as two messengers, one polycistronic (nasABC; nasA encodes Nas) and one monocistronic (nasD; codes for NiR). Here we report the Hfx mediterranei growth as well as the Nas and NiR activities in presence of high nitrate, nitrite and salt concentrations, using different approaches such as physiological experiments and enzymatic activities assays. The nasA and nasD expression profiles are also analysed by real-time quantitative PCR. The results presented reveal that the assimilatory nitrate/nitrite pathway in Hfx mediterranei takes place even if the salt concentration is higher than those usually present in the environments where this microorganism inhabits. This haloarchaeon grows in presence of 2 M nitrate or 50 mM nitrite, which are the highest nitrate and nitrite concentrations described from a prokaryotic microorganism. Therefore, it could be attractive for bioremediation applications in sewage plants where high salt, nitrate and nitrite concentrations are detected in wastewaters and brines.
Authors:
Rosa María Martínez-Espinosa; Belén Lledó; Frutos C Marhuenda-Egea; Susana Díaz; María José Bonete
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-11
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  13     ISSN:  1433-4909     ISO Abbreviation:  Extremophiles     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-03     Completed Date:  2009-11-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  785-92     Citation Subset:  IM; S    
Affiliation:
División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap. 99, 03080, Alicante, Spain.
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MeSH Terms
Descriptor/Qualifier:
Archaeal Proteins / genetics*,  metabolism*
Base Sequence
DNA Primers / genetics
DNA, Archaeal / genetics
Gene Expression
Genes, Archaeal*
Haloferax mediterranei / genetics*,  growth & development,  metabolism*
Nitrate Reductase / genetics*,  metabolism*
Nitrates / metabolism
Nitrite Reductases / genetics*,  metabolism*
Nitrites / metabolism
Salinity
Chemical
Reg. No./Substance:
0/Archaeal Proteins; 0/DNA Primers; 0/DNA, Archaeal; 0/Nitrates; 0/Nitrites; EC 1.7.-/Nitrite Reductases; EC 1.7.99.4/Nitrate Reductase

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