Document Detail

NO signaling in exercise training-induced anti-apoptotic effects in human neutrophils.
MedLine Citation:
PMID:  21195695     Owner:  NLM     Status:  MEDLINE    
Short-lived neutrophils play a predominant role in innate immunity, the effects of exercise training on neutrophil survival is unclear. In this study, we investigated the underlying mechanisms of training effects on human neutrophil apoptosis. Healthy male subjects were trained on a cycling ergometer for 8 weeks and followed by 4 weeks of detraining. Blood neutrophils were collected before exercise, after training, and after detraining. Comparing with pre-exercise specimens, neutrophils collected after training showed reduced apoptosis rate, which partially returned after detraining. Various intracellular proteins, including iNOS, Mcl-1, A1, Grp78, and IL-8, were upregulated by training, and they remained high after detraining. Upregulated iNOS was closely correlated with these anti-apoptotic molecules in neutrophils. Furthermore, the possible mechanism by which iNOS suppressed apoptosis was explored. Neutrophil apoptosis was accelerated by blocking and retarded by stimulating the endogenous iNOS activity. As an anti-apoptosis mediator of NO signaling, the Mcl-1 level dropped by depletion of the major NO downstream molecule cGMP and such loss of Mcl-1 was avoidable when supplying exogenous NO. Upon activation of NO-cGMP signaling, neutrophils held increased Mcl-1 expression and delayed apoptosis. Collectively, our results suggested that exercise training may retard neutrophil apoptosis by upregulating the iNOS-NO-cGMP-Mcl-1 pathway.
Shu-Hui Su; Chauying J Jen; Hsiun-ing Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-31
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  405     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-07     Completed Date:  2011-03-14     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  58-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Institute of Medical Sciences, College of Medicine, Tzu Chi University, Hualien, Taiwan.
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MeSH Terms
Apoptosis Regulatory Proteins / metabolism
Neutrophils / enzymology,  physiology*
Nitric Oxide / physiology*
Nitric Oxide Synthase Type II / biosynthesis
Signal Transduction
Young Adult
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 10102-43-9/Nitric Oxide; EC protein, human; EC Oxide Synthase Type II

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