Document Detail


NMR studies of muscle glycogen synthesis in insulin-resistant offspring of parents with non-insulin-dependent diabetes mellitus immediately after glycogen-depleting exercise.
MedLine Citation:
PMID:  8643574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To examine the impact of insulin resistance on the insulin-dependent and insulin-independent portions of muscle glycogen synthesis during recovery from exercise, we studied eight young, lean, normoglycemic insulin-resistant (IR) offspring of individuals with non-insulin-dependent diabetes mellitus and eight age-weight matched control (CON) subjects after plantar flexion exercise that lowered muscle glycogen to approximately 25% of resting concentration. After approximately 20 min of exercise, intramuscular glucose 6-phosphate and glycogen were simultaneously monitored with 31P and 13C NMR spectroscopies. The postexercise rate of glycogen resynthesis was nonlinear. Glycogen synthesis rates during the initial insulin independent portion (0-1 hr of recovery) were similar in the two groups (IR, 15.5 +/- 1.3 mM/hr and CON, 15.8 +/- 1.7 mM/hr); however, over the next 4 hr, insulin-dependent glycogen synthesis was significantly reduced in the IR group [IR, 0.1 +/- 0.5 mM/hr and CON, 2.9 +/- 0.2 mM/hr; (P < or = 0.001)]. After exercise there was an initial rise in glucose 6-phosphate concentrations that returned to baseline after the first hour of recovery in both groups. In summary, we found that following muscle glycogen-depleting exercise, IR offspring of parents with non-insulin-dependent diabetes mellitus had (i) normal rates of muscle glycogen synthesis during the insulin-independent phase of recovery from exercise and (ii) severely diminished rates of muscle glycogen synthesis during the subsequent recovery period (2-5 hr), which has previously been shown to be insulin-dependent in normal CON subjects. These data provide evidence that exercise and insulin stimulate muscle glycogen synthesis in humans by different mechanisms and that in the IR subjects the early response to stimulation by exercise is normal.
Authors:
T B Price; G Perseghin; A Duleba; W Chen; J Chase; D L Rothman; R G Shulman; G I Shulman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-17     Completed Date:  1996-07-17     Revised Date:  2013-11-21    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5329-34     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Adult
Analysis of Variance
Blood Glucose / metabolism
Diabetes Mellitus, Type 2 / genetics*
Epinephrine / blood
Female
Glucagon / blood
Glucose / pharmacology
Glucose-6-Phosphate
Glucosephosphates / metabolism
Glycogen / metabolism*
Humans
Hydrogen-Ion Concentration
Infusions, Intravenous
Insulin / administration & dosage,  blood,  pharmacology*
Insulin Resistance*
Lactates / blood
Magnetic Resonance Spectroscopy / methods
Male
Muscle Contraction
Muscle, Skeletal / drug effects,  metabolism,  physiology*
Nuclear Family
Phosphates / metabolism
Phosphocreatine / metabolism
Physical Exertion*
Reference Values
Grant Support
ID/Acronym/Agency:
MO1 RR-00125/RR/NCRR NIH HHS; P30 DK-45735/DK/NIDDK NIH HHS; R01 DK-49230/DK/NIDDK NIH HHS; R01 DK040936/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Glucosephosphates; 0/Insulin; 0/Lactates; 0/Phosphates; 020IUV4N33/Phosphocreatine; 56-73-5/Glucose-6-Phosphate; 9005-79-2/Glycogen; 9007-92-5/Glucagon; IY9XDZ35W2/Glucose; YKH834O4BH/Epinephrine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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