Document Detail


NMR spectroscopic assessment of the structure and dynamic properties of an amphibian antimicrobial peptide (Gaegurin 4) bound to SDS micelles.
MedLine Citation:
PMID:  17394777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure and dynamics of a 37-residue antimicrobial peptide gaegurin 4 (GGN4) isolated from the skin of the native Korean frog, Rana rugosa, was determined in SDS micelles by NMR spectroscopy. The solution structure of the peptide in SDS micelles was determined from 352 NOE-derived distance constraints and 22 backbone torsion angle constraints. Dynamic properties for the amide backbone were characterized by (1)H-(15)N heteronuclear NOE experiments. The structural study revealed two amphipathic helices spanning residues 2-10 and 16-32 and that the helices were connected by a flexible loop. An intraresidue disulfide bridge was formed between residues Cys31 and Cys37 near the C-terminus. The loop region (11-15) connecting the two helices are were slightly more flexible than these helices themselves. From the fact that since there is no contact NOEs between two helices, it is implied that the GGN4 peptide shows an independent motion of both helices which has an angle of about 60 degrees -120 degrees from each other.
Authors:
Sangho Park; Woo-Sung Son; Yong-Jin Kim; Ae-Ran Kwon; Bong-Jin Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry and molecular biology     Volume:  40     ISSN:  1225-8687     ISO Abbreviation:  J. Biochem. Mol. Biol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-30     Completed Date:  2007-04-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9702084     Medline TA:  J Biochem Mol Biol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  261-9     Citation Subset:  IM    
Affiliation:
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amphibians / metabolism*
Animals
Antimicrobial Cationic Peptides / chemistry*,  metabolism*
Hydrogen-Ion Concentration
Ion Channels / chemistry*,  isolation & purification,  metabolism*
Magnetic Resonance Spectroscopy
Micelles*
Molecular Sequence Data
Protein Binding
Protein Structure, Secondary
Sodium Dodecyl Sulfate / metabolism*
Temperature
Chemical
Reg. No./Substance:
0/Antimicrobial Cationic Peptides; 0/Ion Channels; 0/Micelles; 0/gaegurin protein, Rana rugosa; 151-21-3/Sodium Dodecyl Sulfate

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