Document Detail


NMR-spectroscopic analysis of mixtures: from structure to function.
MedLine Citation:
PMID:  21071261     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NMR spectroscopy as a particularly information-rich method offers unique opportunities for improving the structural and functional characterization of metabolomes, which will be essential for advancing the understanding of many biological processes. Whereas traditionally NMR spectroscopy was mostly relegated to the characterization of pure compounds, the past few years have seen a surge of interest in using NMR-spectroscopic techniques for characterizing complex metabolite mixtures. Development of new methods was motivated partly by the realization that using NMR for the analysis of metabolite mixtures can help identify otherwise inaccessible small molecules, for example compounds that are prone to chemical decomposition and thus cannot be isolated. Furthermore, comparative metabolomics and statistical analyses of NMR spectra have proven highly effective at identifying novel and known metabolites that correlate with changes in genotype or phenotype. In this review, we provide an overview of the range of NMR-spectroscopic techniques recently developed for characterizing metabolite mixtures, including methods used in discovery-oriented natural product chemistry, in the study of metabolite biosynthesis and function, or for comparative analyses of entire metabolomes.
Authors:
Ry R Forseth; Frank C Schroeder
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2010-11-09
Journal Detail:
Title:  Current opinion in chemical biology     Volume:  15     ISSN:  1879-0402     ISO Abbreviation:  Curr Opin Chem Biol     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-15     Completed Date:  2011-04-13     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9811312     Medline TA:  Curr Opin Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  38-47     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Automatic Data Processing
Genotype
Humans
Magnetic Resonance Spectroscopy / methods*
Phenotype
Grant Support
ID/Acronym/Agency:
GM008500/GM/NIGMS NIH HHS; GM079571/GM/NIGMS NIH HHS; GM088290/GM/NIGMS NIH HHS; P41 GM079571/GM/NIGMS NIH HHS; P41 GM079571-03S1/GM/NIGMS NIH HHS; P41 GM079571-04/GM/NIGMS NIH HHS; R01 GM088290-01A1/GM/NIGMS NIH HHS; R01 GM088290-03/GM/NIGMS NIH HHS; T32 GM008500/GM/NIGMS NIH HHS
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