Document Detail

NMR solution structure of the RNA-binding peptide from human immunodeficiency virus (type 1) Rev.
MedLine Citation:
PMID:  7599117     Owner:  NLM     Status:  MEDLINE    
NMR spectroscopy has been used to solve the three-dimensional solution structure of a minimal RNA-binding domain of the Rev protein from the human immunodeficiency virus (type 1), an essential regulatory protein for viral replication. The presence of 10 arginine residues in the 17-residue peptide Rev34-50 caused significant problems in assignment of the NMR spectra. To improve spectral resolution, the peptide was synthesized with an alanine replacing a nonessential arginine and with selectively 15N-labeled residues. Contrary to Chou-Fasman modeling predictions an alpha-helix was detected in both water and 20% trifluoroethanol (TFE) and was found to span residues that constitute the RNA-binding and nuclear-localizing domains of Rev. The sequence-specific information provided by the NMR data gives a full description of the solution conformation of Rev34-50 which serves as a template for investigating binding of the peptide to RNA from the Rev response element (RRE). Preliminary modeling suggests that the helix can fit neatly into the expanded major groove of the RRE where interactions between the peptide side chains and the RNA can be identified. These data may aid the construction of a suitable pharmacophore model for the rational design of molecules that block Rev-RNA binding and inhibit HIV replication.
M J Scanlon; D P Fairlie; D J Craik; D R Englebretsen; M L West
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  34     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-08-10     Completed Date:  1995-08-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8242-9     Citation Subset:  IM; X    
Centre for Drug Design and Development, University of Queensland, St Lucia, Australia.
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MeSH Terms
Amino Acid Sequence
Crystallography, X-Ray
Gene Products, rev / chemistry*
HIV-1 / metabolism*
Magnetic Resonance Spectroscopy / methods
Models, Structural
Molecular Sequence Data
Peptide Fragments / chemistry
Peptides / chemical synthesis,  chemistry
Protein Conformation*
RNA-Binding Proteins / chemistry*
rev Gene Products, Human Immunodeficiency Virus
Reg. No./Substance:
0/Gene Products, rev; 0/Peptide Fragments; 0/Peptides; 0/RNA-Binding Proteins; 0/rev Gene Products, Human Immunodeficiency Virus

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