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A NMR guided approach for CsrA-RNA crystallization.
MedLine Citation:
PMID:  23359257     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Structure determination of protein-nucleic acid complexes remains a challenging task. Here we present a simple method for generating crystals of a CsrA-nucleic acid complex, guided entirely by results from nuclear magnetic resonances spectroscopy (NMR) spectroscopy. Using a construct that lacks thirteen non-essential C-terminal residues, efficient binding to DNA could be demonstrated. One CsrA dimer interacts with two DNA oligonucleotides, similar to previous findings with RNA. Furthermore, the NMR study of the CsrA-DNA complex was the basis for successfully homing in on conditions that were suitable for obtaining crystals of the CsrA-DNA complex. Our results may be useful for those cases where RNA in protein-nucleic acid complexes may be replaced by DNA.
Authors:
Leonardus M I Koharudin; Rolf Boelens; Rob Kaptein; Angela M Gronenborn
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-29
Journal Detail:
Title:  Journal of biomolecular NMR     Volume:  -     ISSN:  1573-5001     ISO Abbreviation:  J. Biomol. NMR     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9110829     Medline TA:  J Biomol NMR     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Avenue, BST3/Rm1050, Pittsburgh, PA, 15260, USA.
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