| NMR and gas chromatography studies of lyophilized human brain tumors. | |
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MedLine Citation:
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PMID: 20369789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1H HR MAS (high resolution magic angle spinning) NMR is a promising tool in the in vitro characterisation of brain tumors. Brain tissue samples were collected from patients with intracranial tumors during surgery, frozen and lyophilized. The analysis of solid samples was performed by high-speed rotation (33 kHz) H MAS NMR. The most intense resonances arise from lipids (methylene mid-chain and methyl carbons of fatty acids). 1H MAS spectra obtained from the same histological type of brain tumors are similar; spectra of glioblastomas were different from those of meningiomas. 1H and 13C NMR solution spectra of lipid extracts confirmed the presence of aliphatic chains fatty acids and cholesterol as major constituents. Biochemical information on liberated fatty acid composition was obtained by gas-chromatography (GC). The glioma content of the linoleic acid (18:2n6) was found to be greater, whereas the level of docosahexaenoic acid (22:6n3) was significantly reduced. |
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Authors:
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Ryszard Marsza?ek; Maciej Pisklak; Wies?aw Jankowski; Jacek ?ukaszkiewicz; Dariusz Horszty?ski; Iwona Wawer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta poloniae pharmaceutica Volume: 67 ISSN: 0001-6837 ISO Abbreviation: Acta Pol Pharm Publication Date: 2010 Mar-Apr |
Date Detail:
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Created Date: 2010-04-07 Completed Date: 2010-04-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985167R Medline TA: Acta Pol Pharm Country: Poland |
Other Details:
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Languages: eng Pagination: 129-36 Citation Subset: IM |
Affiliation:
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Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warszawa, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Brain Chemistry Brain Neoplasms / chemistry, metabolism* Chromatography, Gas / methods* Fatty Acids / analysis Freeze Drying Humans Lipids / analysis* Magnetic Resonance Spectroscopy / methods* Male Middle Aged |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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