Document Detail


NMR and gas chromatography studies of lyophilized human brain tumors.
MedLine Citation:
PMID:  20369789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1H HR MAS (high resolution magic angle spinning) NMR is a promising tool in the in vitro characterisation of brain tumors. Brain tissue samples were collected from patients with intracranial tumors during surgery, frozen and lyophilized. The analysis of solid samples was performed by high-speed rotation (33 kHz) H MAS NMR. The most intense resonances arise from lipids (methylene mid-chain and methyl carbons of fatty acids). 1H MAS spectra obtained from the same histological type of brain tumors are similar; spectra of glioblastomas were different from those of meningiomas. 1H and 13C NMR solution spectra of lipid extracts confirmed the presence of aliphatic chains fatty acids and cholesterol as major constituents. Biochemical information on liberated fatty acid composition was obtained by gas-chromatography (GC). The glioma content of the linoleic acid (18:2n6) was found to be greater, whereas the level of docosahexaenoic acid (22:6n3) was significantly reduced.
Authors:
Ryszard Marsza?ek; Maciej Pisklak; Wies?aw Jankowski; Jacek ?ukaszkiewicz; Dariusz Horszty?ski; Iwona Wawer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta poloniae pharmaceutica     Volume:  67     ISSN:  0001-6837     ISO Abbreviation:  Acta Pol Pharm     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-04-07     Completed Date:  2010-04-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985167R     Medline TA:  Acta Pol Pharm     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  129-36     Citation Subset:  IM    
Affiliation:
Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warszawa, Poland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Brain Chemistry
Brain Neoplasms / chemistry,  metabolism*
Chromatography, Gas / methods*
Fatty Acids / analysis
Freeze Drying
Humans
Lipids / analysis*
Magnetic Resonance Spectroscopy / methods*
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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