Document Detail


NMR metabolic fingerprinting based identification of grapevine metabolites associated with downy mildew resistance.
MedLine Citation:
PMID:  19785416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Grapevine (Vitis vinifera ssp. vinifera L.) and grapes have been extensively studied due to their numerous nutritional benefits and health affecting activities. In this study, metabolite fingerprinting of crude leaf extracts, based on (1)H nuclear magnetic resonance (NMR) spectroscopy and multivariate data analyses, has been used for the metabolic characterization of six different grapevine cultivars including downy and powdery mildew resistant 'Regent' and susceptible 'Lemberger' among others. Several two-dimensional (2D)-NMR techniques were also employed leading to the identification of a number of different types of compounds. Principal component analysis (PCA), hierarchical cluster analysis (HCA), and partial least-squares-discriminant analysis (PLS-DA) of the processed (1)H NMR data revealed clear differences among the cultivars. Metabolites responsible for the discrimination in different grapevine cultivars belong to major classes, that is, organic acids, amino acids, carbohydrates, phenylpropanoids and flavonoids. A differentiation of the cultivars based on their resistance to downy mildew infection was also achieved, and metabolites associated with this trait, namely, quercetin-3-O-glucoside and a trans-feruloyl derivative, were identified. On the basis of these results, the distribution of different plant metabolites among the different grapevine cultivars is presented.
Authors:
Kashif Ali; Federica Maltese; Eva Zyprian; Martina Rex; Young Hae Choi; Robert Verpoorte
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2010-06-21     Completed Date:  2010-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9599-606     Citation Subset:  IM    
Affiliation:
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, 2333 CC Leiden, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Immunity, Innate
Magnetic Resonance Spectroscopy
Oomycetes / physiology
Plant Diseases / immunology*,  microbiology
Plant Extracts / analysis*
Vitis / chemistry*,  immunology,  metabolism*
Chemical
Reg. No./Substance:
0/Plant Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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