| NMR 13C-isotopic enrichment experiments to study carbon-partitioning into organic solutes in the red alga Grateloupia doryphora. | |
MedLine Citation:
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PMID: 15061080 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The red alga Grateloupia doryphora Montagne (Howe) (Cryptonemiales, Halymeniaceae) was used as a model to investigate the effects of changes in seawater salinity on the intracellular low-molecular-weight organic compounds. Carbon-partitioning into major organic solutes was followed by 13C nuclear magnetic resonance (NMR) spectroscopy on living algae incubated in NaH13CO3-enriched seawater, and by high resolution 1H and 13C NMR experiments performed on 13C-enriched algal extracts. NMR and high performance liquid chromatography (HPLC) analyses both demonstrated that floridoside level was the most affected by changes in salinity: it rose under the hypersaline treatment and decreased under hyposaline one. Moreover, at low salinity, the high labeling of floridoside (45.3% 13C-enrichment for C1) together with its low concentrations both provided evidence of great increase in the de novo biosynthesis and turnover rate. Our experiments also demonstrated a high incorporation of photosynthetic carbon into amino acids, especially glutamate, under hypoosmotic conditions. On the other hand, isethionic acid and N-methyl-methionine sulfoxide were only partly labeled, which indicates they do not directly derive from carbon photoassimilation. In algae exposed to high salinity, elevated concentrations of floridoside coupled to a low labeling (9.4%) were observed. These results suggest that hyperosmotic conditions stimulated floridoside biosynthesis from endogen storage products rather than from carbon assimilation through photosynthesis. |
Authors:
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Christelle Simon-Colin; Nelly Kervarec; Roger Pichon; Eric Deslandes |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology and biochemistry : PPB / Société française de physiologie végétale Volume: 42 ISSN: 0981-9428 ISO Abbreviation: Plant Physiol. Biochem. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-04-05 Completed Date: 2004-05-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882449 Medline TA: Plant Physiol Biochem Country: France |
Other Details:
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Languages: eng Pagination: 21-6 Citation Subset: IM |
Affiliation:
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Laboratoire d'Ecophysiologie et de Biotechnologie des Halophytes et des Algues Marines (LEBHAM), Université de Bretagne Occidentale-IUEM, Technopôle Brest-Iroise, 29280 Plouzané, France. christelle.simon@univ-brest.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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Algae, Red
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metabolism* Amino Acids / chemistry, metabolism Carbon Isotopes Glycerol / analogs & derivatives*, metabolism Isethionic Acid / metabolism Lactic Acid / metabolism Nuclear Magnetic Resonance, Biomolecular / methods* Osmolar Concentration Seawater Sodium Bicarbonate / chemistry, metabolism* Sodium Chloride / chemistry Sulfoxides / chemistry, metabolism Water-Electrolyte Balance / physiology |
| Chemical | |
Reg. No./Substance:
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0/2-galactopyranosylglycerol; 0/Amino Acids; 0/Carbon Isotopes; 0/Sulfoxides; 107-36-8/Isethionic Acid; 144-55-8/Sodium Bicarbonate; 50-21-5/Lactic Acid; 56-81-5/Glycerol; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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