| NIX induces mitochondrial autophagy in reticulocytes. | |
| | |
MedLine Citation:
|
PMID: 18623629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The controlled elimination of defective mitochondria is necessary for the health of long-lived post-mitotic cells, like cardiomyocytes and neurons. Mitochondrial elimination also occurs during the course of normal development, in lens epithelial and erythroid cells. Strikingly, at the final stage of erythroid cell maturation, newly formed erythrocytes, also known as reticulocytes, eliminate their entire cohort of mitochondria. We have employed this model to investigate the mechanism of programmed mitochondrial clearance. NIX (BNIP3L) is a Bcl-2-related protein that is upregulated during terminal erythroid differentiation. NIX-deficient reticulocytes have a significant defect of mitochondrial clearance. Consistent with the ability of NIX to cause mitochondrial depolarization, we show that mitochondria are depolarized in wild type but not NIX deficient reticulocytes. NIX does not function through established proapoptotic pathways, nor does it mediate the induction of autophagy in erythroid cells. Rather, NIX is required for the selective incorporation of mitochondria into autophagosomes. Elucidation of the mechanism of this effect will improve our understanding of the role of autophagy in the maintenance of cellular homeostasis. |
| | |
Authors:
|
Ji Zhang; Paul A Ney |
Related Documents
:
|
19832699 - Mitochondrial targeting of cyclosporin a enables selective inhibition of cyclophilin-d ... 19671539 - Possible role of mitochondrial remodelling on cellular triacylglycerol accumulation. 7811009 - Effect of fialuridine on replication of mitochondrial dna in cem cells and in human hep... 19285479 - Role of mitochondria-associated hexokinase ii in cancer cell death induced by 3-bromopy... 1610369 - Micelles of poly(oxyethylene)-poly(oxypropylene) block copolymer (pluronic) as a tool f... 11041519 - Germline passage of mitochondria: quantitative considerations and possible embryologica... 7846129 - Apoptosis and cancer chemotherapy. 2738159 - Increased sulfation of glycoconjugates by cultured nasal epithelial cells from patients... 21030759 - Tumor cell apoptosis induced by nanoparticle conjugate in combination with radiation th... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Autophagy Volume: 4 ISSN: 1554-8635 ISO Abbreviation: Autophagy Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-07-11 Completed Date: 2008-07-23 Revised Date: 2011-06-30 |
Medline Journal Info:
|
Nlm Unique ID: 101265188 Medline TA: Autophagy Country: United States |
Other Details:
|
Languages: eng Pagination: 354-6 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, St. Jude Children's Hospital, Memphis, Tennessee USA. paul.ney@stjude.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Autophagy / physiology* Cell Differentiation Erythropoiesis Membrane Proteins / genetics, physiology* Mice Mice, Knockout Mitochondria / physiology* Mitochondrial Proteins / genetics, physiology* Phagosomes / physiology Reticulocytes / physiology*, ultrastructure Ubiquitination bcl-2 Homologous Antagonist-Killer Protein / physiology bcl-2-Associated X Protein / physiology |
| Grant Support | |
ID/Acronym/Agency:
|
R21 DK074519-01/DK/NIDDK NIH HHS; R21 DK074519-02/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bak1 protein, mouse; 0/Bax protein, mouse; 0/Membrane Proteins; 0/Mitochondrial Proteins; 0/Nix protein, mouse; 0/bcl-2 Homologous Antagonist-Killer Protein; 0/bcl-2-Associated X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simultaneous single-tube PCR-based assay for the direct identification of the five most common menin...
Next Document: Antioxidative defense parameters in acute and chronic cholecystitis