Document Detail


NIR-Emitting Quantum Dot-Encoded Microbeads through Membrane Emulsification for Multiplexed Immunoassays.
MedLine Citation:
PMID:  23463727     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
NIR-emitting CdSeTe/CdS/ZnS core/shell/shell QD-encoded microbeads are combined with common flow cytometry with one laser for multiplexed detection of hepatitis B virus (HBV). A facile one-pot synthetic route is developed to prepare CdSeTe/CdS/ZnS core/shell/shell QDs with high photoluminescence quantum yield and excellent stability in liquid paraffin, and a Shirasu porous glass (SPG) membrane emulsification technique is applied to incorporate the QDs into polystyrene-maleic anhydride (PSMA) microbeads to obtain highly fluorescent QD-encoded microbeads. The relatively wide NIR photoluminescence full width half maximum of the CdSeTe/CdS/ZnS QDs is used to develop a 'single wavelength' encoding method to obtain different optical codes by changing the wavelengh and emission intensity of the QDs incorporated into the microbeads. Moreover, a detection platform combining NIR-emitting CdSeTe/CdS/ZnS QD-encoded microbeads and Beckman Coulter FC 500 flow cytometry with one laser of 488 nm is successfully used to conduct a 2-plex hybridization assay for hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and a 3-plex hybridization assay for hepatitis B surface antibody (HBsAb), hepatitis B e antibody (HBeAb), and hepatitis B core antibody (HBcAb), which suggests the promising application of NIR QD-encoded microbeads for multiplex immunoassays.
Authors:
Xiebing Wang; Gang Wang; Wanwan Li; Bingxia Zhao; Bin Xing; Yuankui Leng; Hongjing Dou; Kang Sun; Lisong Shen; Xiangliang Yuan; Jiyu Li; Kun Sun; Junsong Han; Huasheng Xiao; Yue Li; Peng Huang; Xiaoyuan Chen
Related Documents :
22849297 - Expression and characterization of recombinant interleukin-21 receptor and its targetin...
2411667 - Anaphylactoid reactions to plasma substitutes.
22544677 - Immunohistochemical detection of intra-neuronal vzv proteins in snap-frozen human gangl...
9729587 - Budesonide affects allergic mucociliary dysfunction.
6944297 - Monoclonal antibodies defining serologically distinct hla-d/dr related ia-like antigens...
7843627 - Soluble forms of p55-il-2r alpha, cd8, and cd30 molecules as markers of lymphoid cell a...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-6
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  -     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cancer-related self-efficacy following a consultation with an oncologist.
Next Document:  Intensive mode delivery of a neuroanatomy unit: Lower final grades but higher student satisfaction.