| A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. | |
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MedLine Citation:
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PMID: 15371535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polycystic kidney disease and related syndromes involve dysregulation of cell proliferation in conjunction with ciliary defects. The relationship between cilia and cell cycle is enigmatic, but it may involve regulation by the NIMA-family of kinases (Neks). We previously showed that the Nek Fa2p is important for ciliary function and cell cycle in Chlamydomonas. We now show that Fa2p localizes to an important regulatory site at the proximal end of cilia in both Chlamydomonas and a mouse kidney cell line. Fa2p also is associated with the proximal end of centrioles. Its localization is dynamic during the cell cycle, following a similar pattern in both cell types. The cell cycle function of Fa2p is kinase independent, whereas its ciliary function is kinase dependent. Mice with mutations in Nek1 or Nek8 have cystic kidneys; therefore, our discovery that a member of this phylogenetic group of Nek proteins is localized to the same sites in Chlamydomonas and kidney epithelial cells suggests that Neks play conserved roles in the coordination of cilia and cell cycle progression. |
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Authors:
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Moe R Mahjoub; M Qasim Rasi; Lynne M Quarmby |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-09-15 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 15 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-10-26 Completed Date: 2005-04-08 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 5172-86 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cell Cycle Proteins / chemistry, physiology* Cell Line Centrioles / metabolism, ultrastructure Chlamydomonas / metabolism* Cilia / metabolism* DNA, Complementary / metabolism Epitopes / chemistry Fluorescent Antibody Technique, Indirect Green Fluorescent Proteins / metabolism Immunoblotting Kidney / metabolism*, pathology Mice Mitosis Mutation Protein Kinases / metabolism Protein Structure, Tertiary Protein-Serine-Threonine Kinases / biosynthesis*, chemistry, metabolism, physiology* Protein-Tyrosine Kinases / metabolism Signal Transduction Subcellular Fractions / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA, Complementary; 0/Epitopes; 147336-22-9/Green Fluorescent Proteins; EC 2.7.-/Protein Kinases; EC 2.7.1.-/NIMA protein kinase; EC 2.7.1.37/Nek8 protein, mouse; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
| Comments/Corrections | |
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