| NGF overexpression and distribution in cortical thymic epithelial cells of mice with experimentally-induced leukemia. | |
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MedLine Citation:
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PMID: 16634528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Thymic epithelial cells often form lymphoid-epithelial cell (LEC) complexes, thought to contribute both to normal T-cell differentiation and to leukemogenesis. The distribution of the nerve growth factor (NGF) and NGF immunoreactivity modulation of complex-forming thymus epithelial cells were studied in mice with experimental acute L1210 leukemia. MATERIALS AND METHODS: Light and electron microscopic methods and cell separation techniques were applied. RESULTS: Immunoperoxidase and immunogold labelling showed subcapsular and subseptal overexpression of NGF by epithelial cells in leukemic thymus. NGF immunopositive epithelial cells were closely associated with lymphoid cells. The increased immunoreactivity of epithelial cells correlated with LEC complex formation, including thymic nurse cell-like structures and rosettes in the external cortex. CONCLUSION: These results provide new structural and immunocytochemical evidence for intimate contact between NGF-producing epithelial cells and lymphoid cells and suggest that NGF immunoreactive LEC complexes are involved in thymic microenvironmental reorganization during leukemogenesis. |
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Authors:
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T S Marinova; M Markova; L Aloe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: In vivo (Athens, Greece) Volume: 20 ISSN: 0258-851X ISO Abbreviation: In Vivo Publication Date: 2006 Mar-Apr |
Date Detail:
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Created Date: 2006-04-25 Completed Date: 2006-05-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8806809 Medline TA: In Vivo Country: Greece |
Other Details:
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Languages: eng Pagination: 259-64 Citation Subset: IM |
Affiliation:
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Department of Biology, Medical Faculty, Medical University of Sofia, Bulgaria. tmarin@medfac.acad.bg |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Separation Epithelial Cells / metabolism, ultrastructure Female Immunoenzyme Techniques Immunohistochemistry Leukemia L1210 / metabolism*, pathology Leukemia, Lymphoid / metabolism*, pathology Male Mice Mice, Inbred DBA Microscopy, Electron, Transmission Nerve Growth Factor / metabolism* Organelles / ultrastructure Thymus Gland / metabolism*, pathology Tumor Markers, Biological / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological; 9061-61-4/Nerve Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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