Document Detail

NGF overexpression and distribution in cortical thymic epithelial cells of mice with experimentally-induced leukemia.
MedLine Citation:
PMID:  16634528     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Thymic epithelial cells often form lymphoid-epithelial cell (LEC) complexes, thought to contribute both to normal T-cell differentiation and to leukemogenesis. The distribution of the nerve growth factor (NGF) and NGF immunoreactivity modulation of complex-forming thymus epithelial cells were studied in mice with experimental acute L1210 leukemia. MATERIALS AND METHODS: Light and electron microscopic methods and cell separation techniques were applied. RESULTS: Immunoperoxidase and immunogold labelling showed subcapsular and subseptal overexpression of NGF by epithelial cells in leukemic thymus. NGF immunopositive epithelial cells were closely associated with lymphoid cells. The increased immunoreactivity of epithelial cells correlated with LEC complex formation, including thymic nurse cell-like structures and rosettes in the external cortex. CONCLUSION: These results provide new structural and immunocytochemical evidence for intimate contact between NGF-producing epithelial cells and lymphoid cells and suggest that NGF immunoreactive LEC complexes are involved in thymic microenvironmental reorganization during leukemogenesis.
T S Marinova; M Markova; L Aloe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  20     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-04-25     Completed Date:  2006-05-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  259-64     Citation Subset:  IM    
Department of Biology, Medical Faculty, Medical University of Sofia, Bulgaria.
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MeSH Terms
Cell Separation
Epithelial Cells / metabolism,  ultrastructure
Immunoenzyme Techniques
Leukemia L1210 / metabolism*,  pathology
Leukemia, Lymphoid / metabolism*,  pathology
Mice, Inbred DBA
Microscopy, Electron, Transmission
Nerve Growth Factor / metabolism*
Organelles / ultrastructure
Thymus Gland / metabolism*,  pathology
Tumor Markers, Biological / metabolism
Reg. No./Substance:
0/Tumor Markers, Biological; 9061-61-4/Nerve Growth Factor

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