Document Detail


NGF-induced cytotoxicity in PC12 cells in a hypoglycemic environment.
MedLine Citation:
PMID:  9721921     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Surprisingly, we observed that nerve growth factor (NGF) potentiated death of PC12 cells induced by glucose withdrawal, although NGF is widely believed to exert its protective role against several types of cell death. Since either glucose withdrawal or NGF treatment increases intracellular calcium levels of target cells in many cases, we hypothesized that further increase of intracellular calcium by NGF may be a determinant factor in the NGF-mediated cell death. To test this hypothesis, we examined the effect of NGF on cell death pharmacologically by measuring cell viability and traced the changes of intracellular calcium in various conditions using a confocal laser microscope. NGF promoted cell death under a glucose-deprived condition in a manner dependent on extracellular calcium, and nifedipine, but not ryanodine, could partially block the cell death. NGF treatment augmented further intracellular calcium that had been elevated by glucose withdrawal, the event that nifedipine could block. In this study, therefore, we tentatively concluded that NGF potentiates cell death of starved PC12 cells by accelerating the initial increase of intracellular calcium through activation of a dihydropyridine-sensitive calcium channel.
Authors:
J M Chung; J H Hong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  9     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-11-18     Completed Date:  1998-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2495-500     Citation Subset:  IM    
Affiliation:
Department of Biology, College of Natural Sciences, Ewha Womans University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / physiology
Calcium Channel Blockers / pharmacology
Calcium Channels / drug effects,  metabolism
Calcium Signaling / drug effects,  physiology*
Cell Survival / drug effects
Culture Media
Fluorescent Dyes
Glucose / pharmacology
Hypoglycemia / pathology*
Microscopy, Confocal
Nerve Growth Factors / toxicity*
Nifedipine / pharmacology
PC12 Cells
Rats
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Calcium Channels; 0/Culture Media; 0/Fluorescent Dyes; 0/Nerve Growth Factors; 21829-25-4/Nifedipine; 50-99-7/Glucose; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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