Document Detail

NGF gene expression in dividing and non-dividing cells from AAV-derived constructs.
MedLine Citation:
PMID:  9566618     Owner:  NLM     Status:  MEDLINE    
NGF expression in COS cells when driven by pTR.NGF (CMV promoter, AAV TRs) was more effective than either pc.NGF (CMV promoter, no AAV TRs) or dlk.NGF (AAV promoters and TRs). This NGF was able to differentiate PC12 cells. Differentiated PC12 cells transfected with pTR.NGF released NGF into medium. The fraction of pTR.NGF transfected PC12 cells that extended neurite-like processes 7 days post-transfection was similar to the transfection efficiency, suggesting that transfected cells were selectively differentiated by locally released NGF. pTR.NGF-transfected primary cultures of either neurons or glia did not express exogenous NGF. These results indicate that NGF can be released by dividing and non-dividing cells, but not neonatally derived brain cells.
S Wang; W J Millard; E M Meyer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  23     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-19     Completed Date:  1998-06-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  779-86     Citation Subset:  IM    
Department of Pharmacology, University of Florida, Gainesville 32610, USA.
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MeSH Terms
COS Cells
Cell Division
Cells, Cultured
Cytomegalovirus / genetics
Genetic Vectors
Nerve Growth Factors / biosynthesis*
Neurites / physiology*
Neuroglia / cytology,  physiology*
Neurons / cytology,  physiology*
PC12 Cells
Promoter Regions, Genetic
Recombinant Proteins / biosynthesis
Grant Support
Reg. No./Substance:
0/Nerve Growth Factors; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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