Document Detail

NGF-differentiated and undifferentiated PC12 cells vary in induction of apoptosis by ethanol.
MedLine Citation:
PMID:  10372659     Owner:  NLM     Status:  MEDLINE    
The present study was undertaken to determine whether the neurotoxic effects of ethanol vary between undifferentiated and differentiated neurons. For this study, untreated rat pheochromocytoma (PC12) cells and PC12 cells treated for 8-10 days with nerve growth factor (NGF) were used as models of undifferentiated and differentiated neurons, respectively. Treatment of differentiated PC12 cells with 150 mM ethanol resulted in a loss of cells whereas a similar treatment of undifferentiated cells had no effect. In contrast, 50 mM ethanol enhanced apoptosis initiated by serum withdrawal in undifferentiated cells while a similar response in the differentiated cells required 150 mM ethanol. This study demonstrates that undifferentiated and differentiated neuronal cells differ in their sensitivity to the neurotoxic actions of ethanol.
J Oberdoerster; R A Rabin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  64     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1999  
Date Detail:
Created Date:  1999-06-25     Completed Date:  1999-06-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  PL 267-72     Citation Subset:  IM    
Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 14214-3000, USA.
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MeSH Terms
Apoptosis / drug effects*
Cell Death / drug effects
Cell Differentiation / drug effects
Cell Survival / drug effects
Culture Media / pharmacology
Culture Media, Serum-Free / pharmacology
DNA / drug effects,  metabolism
DNA Fragmentation / drug effects
Ethanol / adverse effects*
Nerve Growth Factors / pharmacology*
PC12 Cells / cytology,  drug effects,  physiology
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Culture Media, Serum-Free; 0/Nerve Growth Factors; 64-17-5/Ethanol; 9007-49-2/DNA

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