| NF-kappaB inhibitors from Brucea javanica exhibiting intracellular effects on reactive oxygen species. | |
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MedLine Citation:
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PMID: 20944100 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Brucea javanica was studied to identify nuclear factor kappaB (NF-κB) inhibitors exhibiting reactive oxygen species (ROS) intracellular amplification. MATERIAL AND METHODS: Eight compounds were evaluated for selective cytotoxicity using HT-29, HeLa, and HL-60 cells, and in a NF-κB assay. Active compounds were then tested using ROS and mitochondria transmembrane potential (MTP) assays. NF-κB and nuclear factor activated T-cell (NFAT) translocation were also assessed using their respective whole cell assays. RESULTS: Bruceajavanone B, bruceantin, bruceine A, (-)-hydnocarpin, and chrysoeriol exhibited cytotoxic potential and NF-κB p65 inhibition. Chrysoeriol exhibited selective cytotoxicity against leukemia cells with greater potency and also showed an ability to up-regulate NFAT transcriptional pathways through the amplification of intracellular ROS, in the presence of H2O2, to a greater degree than bruceantin and bruceine. CONCLUSION: Chrysoeriol selectively kills leukemic cells and potentiates the amplification of ROS levels. Therefore, chrysoeriol could serve as a potential chemotherapeutic modifier for leukemia chemotherapy since leukemia cells have a higher susceptibility to elevated ROS levels. |
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Authors:
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Jeong-Ah Kim; Edward K Lau; Li Pan; Esperanza J Carcache De Blanco |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-10-14 Completed Date: 2010-11-10 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 3295-300 Citation Subset: IM |
Affiliation:
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Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Brucea
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chemistry* Cell Line, Tumor Flavonoids / pharmacology Flavonolignans / pharmacology Humans Membrane Potential, Mitochondrial / drug effects NF-kappa B / antagonists & inhibitors*, metabolism NFATC Transcription Factors / drug effects, metabolism Plant Extracts / pharmacology* Quassins / pharmacology Reactive Oxygen Species / metabolism* Triterpenes / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA125066-03/CA/NCI NIH HHS; P01-CA125066/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Flavonoids; 0/Flavonolignans; 0/NF-kappa B; 0/NFATC Transcription Factors; 0/Plant Extracts; 0/Quassins; 0/Reactive Oxygen Species; 0/Triterpenes; 161043-67-0/bruceajavanin B; 25514-31-2/bruceine A; 41451-75-6/bruceantin; 491-71-4/chrysoeriol; 51419-48-8/hydnocarpin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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