Document Detail

NF-kappaB and AP-1 are required for cyclo-oxygenase 2 gene expression in amnion epithelial cell line (WISH).
MedLine Citation:
PMID:  10825375     Owner:  NLM     Status:  MEDLINE    
Prostaglandins are known to play an important role in human labour and are used clinically to induce labour onset. Cytokines, e.g. interleukin 1 beta (IL-1beta), are up-regulated in the amniotic fluid late in gestation and can increase prostaglandin production through the expression of cyclo-oxygenase 2 (COX-2), the prostaglandin synthetic isoform involved in human labour. We demonstrate in immortalized amnion epithelial (WISH) cells, that IL-1beta causes increased transcription of the COX-2 gene. Luciferase reporter constructs with site-directed mutagenesis of the two NF-kappaB sites and an AP-1 site in the COX-2 promoter showed reduced expression of luciferase in transient transfection studies. This suggests that the binding of transcription factors to these sites is essential for the regulation of COX-2 transcription in IL-1beta-treated WISH cells.
V C Allport; D M Slater; R Newton; P R Bennett
Related Documents :
14988445 - Quercetin metabolites downregulate cyclooxygenase-2 transcription in human lymphocytes ...
10825375 - Nf-kappab and ap-1 are required for cyclo-oxygenase 2 gene expression in amnion epithel...
18778395 - Cox-2 expression is unexpectedly high in viable colorectal mucosal cells: is there life...
16949875 - Peripheral formalin injection induces long-lasting increases in cyclooxygenase 1 expres...
19258345 - Mitogen-activated protein kinase (mapk) pathways mediate embryonic responses to culture...
2361015 - Cloning and expression of cdna and genomic clones encoding three delayed rectifier pota...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular human reproduction     Volume:  6     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-25     Completed Date:  2000-07-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  561-5     Citation Subset:  IM    
Imperial College School of Medicine, Institute of Obstetrics and Gynaecology, Queen Charlotte's and Chelsea Hospital, London, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amnion / cytology,  drug effects,  metabolism*
Binding Sites
Blotting, Western
Cell Line / drug effects
Cyclooxygenase 2
Dinoprostone / metabolism
Epithelial Cells / drug effects,  metabolism
Gene Expression Regulation
Genes, Reporter
Interleukin-1 / metabolism,  pharmacology
Isoenzymes / drug effects,  genetics*,  metabolism
Membrane Proteins
Mutagenesis, Site-Directed
NF-kappa B / genetics,  metabolism*
Promoter Regions, Genetic
Prostaglandin-Endoperoxide Synthases / drug effects,  genetics*,  metabolism
Recombinant Proteins / genetics,  metabolism
Transcription Factor AP-1 / genetics,  metabolism*
Reg. No./Substance:
0/Interleukin-1; 0/Isoenzymes; 0/Membrane Proteins; 0/NF-kappa B; 0/Recombinant Proteins; 0/Transcription Factor AP-1; 363-24-6/Dinoprostone; EC 2; EC protein, human; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  beta-endorphin inhibits the production of interleukin-8 by human chorio-decidual cells in culture.
Next Document:  Segregation of sex chromosomes in spermatozoa of 46,XY/47,XXY men by multicolour fluorescence in-sit...