Document Detail


NF-kappaB and AP-1 are required for cyclo-oxygenase 2 gene expression in amnion epithelial cell line (WISH).
MedLine Citation:
PMID:  10825375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prostaglandins are known to play an important role in human labour and are used clinically to induce labour onset. Cytokines, e.g. interleukin 1 beta (IL-1beta), are up-regulated in the amniotic fluid late in gestation and can increase prostaglandin production through the expression of cyclo-oxygenase 2 (COX-2), the prostaglandin synthetic isoform involved in human labour. We demonstrate in immortalized amnion epithelial (WISH) cells, that IL-1beta causes increased transcription of the COX-2 gene. Luciferase reporter constructs with site-directed mutagenesis of the two NF-kappaB sites and an AP-1 site in the COX-2 promoter showed reduced expression of luciferase in transient transfection studies. This suggests that the binding of transcription factors to these sites is essential for the regulation of COX-2 transcription in IL-1beta-treated WISH cells.
Authors:
V C Allport; D M Slater; R Newton; P R Bennett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular human reproduction     Volume:  6     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-25     Completed Date:  2000-07-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  561-5     Citation Subset:  IM    
Affiliation:
Imperial College School of Medicine, Institute of Obstetrics and Gynaecology, Queen Charlotte's and Chelsea Hospital, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Amnion / cytology,  drug effects,  metabolism*
Binding Sites
Blotting, Western
Cell Line / drug effects
Cyclooxygenase 2
Dinoprostone / metabolism
Epithelial Cells / drug effects,  metabolism
Gene Expression Regulation
Genes, Reporter
Humans
Interleukin-1 / metabolism,  pharmacology
Isoenzymes / drug effects,  genetics*,  metabolism
Membrane Proteins
Mutagenesis, Site-Directed
NF-kappa B / genetics,  metabolism*
Promoter Regions, Genetic
Prostaglandin-Endoperoxide Synthases / drug effects,  genetics*,  metabolism
Recombinant Proteins / genetics,  metabolism
Transcription Factor AP-1 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Isoenzymes; 0/Membrane Proteins; 0/NF-kappa B; 0/Recombinant Proteins; 0/Transcription Factor AP-1; 363-24-6/Dinoprostone; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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