Document Detail

NF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.
MedLine Citation:
PMID:  1309887     Owner:  NLM     Status:  MEDLINE    
A cDNA encoding NF-IL6, an interleukin-6 (IL-6)-regulated human nuclear factor of the C/EBP family, is demonstrated to complement the transactivation function of E1A. The endogenous NF-IL6 level varies according to cell type and correlates positively with an IL-6-regulated cellular E1A-substituting activity that was described recently (J.M. Spergel and S. Chen-Kiang, Proc. Natl. Acad. Sci. USA 88:6472-6476, 1991). When expressed by transfection in cells which contain low levels of NF-IL6 and are incapable of complementing the function of E1A proteins, NF-IL6 also transactivates the E1A-responsive E2ae and E1B promoters, to the same magnitude as E1A. Activation by NF-IL6 is concentration dependent and sequence specific: mutational studies of the E2ae promoter suggest that the promoter-proximal NF-IL6 recognition site functions as a dominant negative regulatory site whereas the promoter-distal NF-IL6 recognition site is positively regulated at low NF-IL6 concentrations and negatively regulated when the NF-IL6 level is high. Consistent with these functions, NF-IL6 alone is sufficient to complement an E1A deletion mutant dl312 in viral infection, when expressed at appropriate concentrations. These results identify NF-IL6 as a sequence-specific cellular nuclear factor which regulates E1A-responsive genes in the absence of E1A.
J M Spergel; W Hsu; S Akira; B Thimmappaya; T Kishimoto; S Chen-Kiang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-14     Completed Date:  1992-02-14     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1021-30     Citation Subset:  IM    
Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029.
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MeSH Terms
Adenovirus Early Proteins
Adenoviruses, Human / genetics*
Base Sequence
CCAAT-Enhancer-Binding Proteins
Carcinoma, Hepatocellular
Cell Line
Chloramphenicol O-Acetyltransferase / genetics,  metabolism
DNA-Binding Proteins / metabolism*
Gene Expression Regulation, Viral
Interleukin-6 / physiology
Liver Neoplasms
Molecular Sequence Data
Nuclear Proteins / metabolism*
Oncogene Proteins, Viral / genetics*
Promoter Regions, Genetic*
Repetitive Sequences, Nucleic Acid
Signal Transduction
Trans-Activators / genetics*,  metabolism
Transcription Factors / metabolism*
Transcriptional Activation
Grant Support
Reg. No./Substance:
0/Adenovirus Early Proteins; 0/CCAAT-Enhancer-Binding Proteins; 0/DNA-Binding Proteins; 0/Interleukin-6; 0/Nuclear Proteins; 0/Oligodeoxyribonucleotides; 0/Oncogene Proteins, Viral; 0/Trans-Activators; 0/Transcription Factors; EC O-Acetyltransferase

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