Document Detail

NF-IL6-mediated transcriptional activation of the long terminal repeat of the human immunodeficiency virus type 1.
MedLine Citation:
PMID:  8346247     Owner:  NLM     Status:  MEDLINE    
An upstream control region in the long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) includes a potential negative regulatory element (NRE1). Cotransfecting multimers of a sequence spanning this element with an LTR-CAT construct produced an increase in chloramphenicol acetyltransferase (CAT) activity in Jurkat and HepG2 cells, providing further evidence and support for the existence of an NRE. In screening experiments aimed at identifying those factors that regulate HIV-1 transcription through interactions with the NRE1 region, we isolated a cDNA for NF-IL6. Previous studies have shown that NF-IL6 is a key nuclear factor that activates gene expression in response to interleukin 6. By methylation interference analysis, we have localized the NF-IL6 binding site within the NRE1 region and found that it overlaps an E box that has previously been implicated as the binding element for a negative regulator of HIV-1 expression. Through a database search, we identified an additional consensus binding sequence for NF-IL6 in the LTR of many HIV-1 variants and found that over this sequence, purified NF-IL6 can produce an extended footprint that overlaps one of the binding sites for NF-kappa B. A product of the nf-il6 gene activated transcription from several LTR-CAT constructs in transient transfection assays. Thus, NF-IL6 could play a central role in the control of HIV-1 gene expression and this protein might be a key mediator in signaling pathways where HIV-1 is activated by interleukin 6.
V M Tesmer; A Rajadhyaksha; J Babin; M Bina
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  90     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-09-07     Completed Date:  1993-09-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7298-302     Citation Subset:  IM; X    
Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393.
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MeSH Terms
Base Sequence
Binding Sites
CCAAT-Enhancer-Binding Proteins
Cloning, Molecular
DNA-Binding Proteins / pharmacology*
Gene Expression Regulation, Viral*
HIV Long Terminal Repeat / genetics*
HIV-1 / genetics*
Molecular Sequence Data
Nuclear Proteins / pharmacology*
Oligodeoxyribonucleotides / chemistry
Transcription, Genetic
Transcriptional Activation*
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Oligodeoxyribonucleotides

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