Document Detail


NF-IL6-mediated transcriptional activation of the long terminal repeat of the human immunodeficiency virus type 1.
MedLine Citation:
PMID:  8346247     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An upstream control region in the long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) includes a potential negative regulatory element (NRE1). Cotransfecting multimers of a sequence spanning this element with an LTR-CAT construct produced an increase in chloramphenicol acetyltransferase (CAT) activity in Jurkat and HepG2 cells, providing further evidence and support for the existence of an NRE. In screening experiments aimed at identifying those factors that regulate HIV-1 transcription through interactions with the NRE1 region, we isolated a cDNA for NF-IL6. Previous studies have shown that NF-IL6 is a key nuclear factor that activates gene expression in response to interleukin 6. By methylation interference analysis, we have localized the NF-IL6 binding site within the NRE1 region and found that it overlaps an E box that has previously been implicated as the binding element for a negative regulator of HIV-1 expression. Through a database search, we identified an additional consensus binding sequence for NF-IL6 in the LTR of many HIV-1 variants and found that over this sequence, purified NF-IL6 can produce an extended footprint that overlaps one of the binding sites for NF-kappa B. A product of the nf-il6 gene activated transcription from several LTR-CAT constructs in transient transfection assays. Thus, NF-IL6 could play a central role in the control of HIV-1 gene expression and this protein might be a key mediator in signaling pathways where HIV-1 is activated by interleukin 6.
Authors:
V M Tesmer; A Rajadhyaksha; J Babin; M Bina
Related Documents :
24266967 - Repression of farnesoid x receptor contributes to biliary injuries of liver grafts thro...
23624977 - Alternative splicing of transcription factors in plant responses to low temperature str...
8623547 - Proteolytic activity of human immunodeficiency virus vpr- and vpx-protease fusion prote...
14762397 - Reciprocal regulation of the nuclear factor of activated t cells and hiv-1.
15528387 - Polymorphisms in the fc epsilon ri beta promoter region affecting transcription activit...
12577317 - Expression of interleukin-1 beta and interleukin-1 receptor antagonist in oxldl-treated...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  90     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-09-07     Completed Date:  1993-09-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7298-302     Citation Subset:  IM; X    
Affiliation:
Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites
CCAAT-Enhancer-Binding Proteins
Cloning, Molecular
DNA-Binding Proteins / pharmacology*
Gene Expression Regulation, Viral*
HIV Long Terminal Repeat / genetics*
HIV-1 / genetics*
Humans
Molecular Sequence Data
Nuclear Proteins / pharmacology*
Oligodeoxyribonucleotides / chemistry
Transcription, Genetic
Transcriptional Activation*
Chemical
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Oligodeoxyribonucleotides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of an intronless collagen gene family in the marine sponge Microciona prolifera.
Next Document:  Pleiotropic effect of the human T-cell leukemia virus Tax protein on the DNA binding activity of euk...