Document Detail


NDRG2 is one of novel intrinsic factors for regulation of IL-10 production in human myeloid cell.
MedLine Citation:
PMID:  20438703     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
N-myc downstream-regulated gene 2 (NDRG2) implicated in cellular growth and differentiation was previously reported as it is specifically expressed in primary and in vitro-differentiated dendritic cells (DCs) from monocytes and CD34(+) progenitor cells. However, its function has yet to be investigated in DCs. Here, the novel NDRG2 function about modulation of cytokines in DC was observed in this study. The secretion of IL-10 was not found in the monocyte-derived DC cells with high level of NDRG2 expression, but IL-10 was abundantly secreted up to 1ng/ml in the monocyte-derived macrophages with low level of NDRG2 expression, and further confirmed that the expression of IL-10 was dramatically increased in NDRG2-silenced DCs under presence of LPS, and significantly reduced in the NDRG2-overexpressed U937 cells under stimulation of PMA. The secretion of IL-12p70 was significantly reduced in the siNDRG2 introduced DC cells. The intracellular signaling of IL-10 secretion was markedly inhibited by SB203580, inhibitor of p38 MAPK, in the LPS-activated DCs and phosphorylation of p38 MAPK was decreased in the NDRG2 introduced U937 cells under PMA-stimulation. Taken together, NDRG2 might have a pivotal role as one of intrinsic factors for the modulation of p38 MAPK phosphorylation, and subsequently involve in controlling of IL-10 production.
Authors:
Seung-Chul Choi; Kwang Dong Kim; Jong-Tae Kim; Sang-Seok Oh; Sun Young Yoon; Eun Young Song; Hee Gu Lee; Yong-Kyung Choe; Inpyo Choi; Jong-Seok Lim; Jae Wha Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-11
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  396     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-07-07     Revised Date:  2010-07-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  684-90     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Affiliation:
Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Dendritic Cells / metabolism
Humans
Imidazoles / pharmacology
Interleukin-10 / biosynthesis*,  genetics
Lipopolysaccharides
Macrophages / metabolism
Myeloid Cells / metabolism*
Phosphorylation
Protein Kinase Inhibitors / pharmacology
Pyridines / pharmacology
RNA, Small Interfering / genetics
Tumor Suppressor Proteins / genetics,  metabolism*
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism
Chemical
Reg. No./Substance:
0/Imidazoles; 0/Lipopolysaccharides; 0/NDRG2 protein, human; 0/Protein Kinase Inhibitors; 0/Pyridines; 0/RNA, Small Interfering; 0/SB 203580; 0/Tumor Suppressor Proteins; 130068-27-8/Interleukin-10; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases
Comments/Corrections
Erratum In:
Biochem Biophys Res Commun. 2010 Jul 9;397(4):767

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