Document Detail

NDP52, a novel autophagy receptor for ubiquitin-decorated cytosolic bacteria.
MedLine Citation:
PMID:  20104023     Owner:  NLM     Status:  MEDLINE    
Autophagy functions as a cell-autonomous effector mechanism of innate immunity by separating bacteria from cytosolic resources and delivering them for lysosomal destruction. How cytosolic bacteria are targeted for autophagy is incompletely understood. We recently discovered that Salmonella enterica serotype Typhimurium and Streptococcus pyogenes are detected by NDP52 (nuclear dot protein 52 kDa), after these bacteria enter the cytosol of human cells and become decorated with polyubiquitinated proteins. NDP52 binds the bacterial ubiquitin coat as well as ATG8/LC3 and delivers cytosolic bacteria into autophagosomes. In the absence of NDP52 ubiquitin-coated bacteria accumulate outside ATG8/LC3(+) autophagosomes. Cells lacking NDP52 fail to restrict bacterial proliferation, as do cells depleted of TBK1, an IKK family kinase colocalizing with NDP52 at the bacterial surface. Our findings demonstrate the existence of a receptor for the selective autophagy of cytosolic bacteria, suggesting that cells are able to differentiate between antibacterial and other forms of autophagy.
Natalia von Muhlinen; Teresa Thurston; Grigory Ryzhakov; Stuart Bloor; Felix Randow
Related Documents :
7700133 - A quantitative chemiluminescent ribosomal probe method for monitoring adherence of haem...
6983493 - A comparison of regulatory cells from rabbit spleen and appendix.
24590713 - Automated cell-free protein production methods for structural studies.
Publication Detail:
Type:  Journal Article     Date:  2010-02-04
Journal Detail:
Title:  Autophagy     Volume:  6     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-04-13     Completed Date:  2010-07-06     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  288-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Autophagy / physiology*
Cytosol / metabolism
Nuclear Proteins / metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism
Salmonella typhimurium / cytology,  metabolism*
Streptococcus pyogenes / metabolism*,  ultrastructure
Ubiquitin / metabolism*
Grant Support
MC_U105170648//Medical Research Council
Reg. No./Substance:
0/Nuclear Proteins; 0/Ubiquitin; 0/nuclear dot protein 52, human; EC Kinases; EC protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impa...
Next Document:  Celecoxib-induced apoptosis is enhanced by ABT-737 and by inhibition of autophagy in human colorecta...