Document Detail


NDP52, a novel autophagy receptor for ubiquitin-decorated cytosolic bacteria.
MedLine Citation:
PMID:  20104023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Autophagy functions as a cell-autonomous effector mechanism of innate immunity by separating bacteria from cytosolic resources and delivering them for lysosomal destruction. How cytosolic bacteria are targeted for autophagy is incompletely understood. We recently discovered that Salmonella enterica serotype Typhimurium and Streptococcus pyogenes are detected by NDP52 (nuclear dot protein 52 kDa), after these bacteria enter the cytosol of human cells and become decorated with polyubiquitinated proteins. NDP52 binds the bacterial ubiquitin coat as well as ATG8/LC3 and delivers cytosolic bacteria into autophagosomes. In the absence of NDP52 ubiquitin-coated bacteria accumulate outside ATG8/LC3(+) autophagosomes. Cells lacking NDP52 fail to restrict bacterial proliferation, as do cells depleted of TBK1, an IKK family kinase colocalizing with NDP52 at the bacterial surface. Our findings demonstrate the existence of a receptor for the selective autophagy of cytosolic bacteria, suggesting that cells are able to differentiate between antibacterial and other forms of autophagy.
Authors:
Natalia von Muhlinen; Teresa Thurston; Grigory Ryzhakov; Stuart Bloor; Felix Randow
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Publication Detail:
Type:  Journal Article     Date:  2010-02-04
Journal Detail:
Title:  Autophagy     Volume:  6     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-04-13     Completed Date:  2010-07-06     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  288-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autophagy / physiology*
Cytosol / metabolism
Humans
Nuclear Proteins / metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism
Salmonella typhimurium / cytology,  metabolism*
Streptococcus pyogenes / metabolism*,  ultrastructure
Ubiquitin / metabolism*
Grant Support
ID/Acronym/Agency:
MC_U105170648//Medical Research Council
Chemical
Reg. No./Substance:
0/Nuclear Proteins; 0/Ubiquitin; 0/nuclear dot protein 52, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/TBK1 protein, human

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