| NDP kinase 7 is a conserved microtubule-binding protein preferentially expressed in ciliated cells. | |
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MedLine Citation:
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PMID: 20215702 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleoside diphosphate (NDP) kinase is an enzyme that synthesizes the nucleoside triphosphates. In mammals, nine sequences (NDK1-NDK9) have been found with domain(s) homologous to the catalytic domain of NDP kinase, and some of them have been shown to associate with sperm flagella. The present study examines the localization of NDK7, for which little information has been available. Database analysis showed that the NDK7 gene is present in organisms with cilia and flagella. Western blotting analyses of various mouse tissues consistently indicated that NDK7 is preferentially expressed in tissues with motile cilia as well as in sperm. Immunofluorescence microscopy revealed that this protein is localized along the entire length of the TritonX-100-insoluble fraction of sperm flagella, possibly in the axonemes. Unexpectedly, however, NDK7 in tracheal epithelia was found in the cell body but not in cilia. Finally, in vitro co-sedimentation assays using recombinant proteins showed that both mouse and Chlamydomonas NDK7 directly bind to microtubules. |
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Authors:
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Takashi Ikeda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-10 |
Journal Detail:
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Title: Cell structure and function Volume: 35 ISSN: 1347-3700 ISO Abbreviation: Cell Struct. Funct. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-04-22 Completed Date: 2010-07-27 Revised Date: 2011-09-21 |
Medline Journal Info:
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Nlm Unique ID: 7608465 Medline TA: Cell Struct Funct Country: Japan |
Other Details:
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Languages: eng Pagination: 23-30 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Chlamydomonas / enzymology Cilia / enzymology* Flagella / enzymology Mice Microtubules / metabolism* Molecular Sequence Data NM23 Nucleoside Diphosphate Kinases / analysis, genetics, metabolism* Nucleoside-Diphosphate Kinase / analysis, genetics, metabolism* Recombinant Proteins / analysis, genetics, metabolism Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/NM23 Nucleoside Diphosphate Kinases; 0/Recombinant Proteins; EC 2.7.4.6/NDP kinase 7, mouse; EC 2.7.4.6/Nucleoside-Diphosphate Kinase |
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