Document Detail


NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses.
MedLine Citation:
PMID:  2260924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Primary rhesus monkey kidney (MK) cells have long been the cells of choice for isolation and propagation of the human paramyxoviruses (parainfluenza 1, 2, 3, 4A, 4B, and mumps). However, problems with the supply and cost of MK cells and the presence of endogenous viruses, including herpes B virus and SV-5, necessitated a search for an alternative cell line. Continuous cell cultures of human origin (L132, A-549, HuT-292, HEK, G-293, G-401, A-498, A-704, CAKI-1, RD) and simian origin (LLC-MK2, BSC-1, MA-104, Vero) were evaluated for their capacity to support the growth of the human paramyxoviruses, as followed by cytopathic effect, hemadsorption, hemagglutination, and EIA. NCI-H292 (HuT-292) human lung mucoepidermoid carcinoma cells (ATCC # CRL-1848) proved to be the most sensitive line for cultivating all serotypes and strains of the paramyxoviruses. These cells were also shown to be a suitable substitute for MK in primary isolation of paramyxoviruses from clinical specimens. RPMI-1640 with 1.5 micrograms/ml trypsin was the preferred maintenance medium; alternatively, Eagle's MEM supplemented with 1.5 micrograms/ml trypsin and 0.1% ITS was satisfactory. NCI-H292 cells are a continuous line with excellent growth characteristics, although the genetic polyploidy of the cells may limit the number of passages of usable cells.
Authors:
E Castells; V G George; J C Hierholzer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of virology     Volume:  115     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1990  
Date Detail:
Created Date:  1991-01-28     Completed Date:  1991-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  277-88     Citation Subset:  IM    
Affiliation:
Respiratory and Enteric Viruses Branch, Centers for Disease Control, Atlanta, Georgia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cytopathogenic Effect, Viral
Hemagglutination Tests
Humans
Immunoenzyme Techniques
Lung / cytology
Paramyxoviridae / growth & development*
Prospective Studies
Tumor Cells, Cultured / microbiology*
Virus Cultivation*
Virus Replication

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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